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Accurate discrimina...
Accurate discrimination of the wake-sleep states of mice using non-invasive whole-body plethysmography
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Bastianini, S (author)
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Alvente, S (author)
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Berteotti, C (author)
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Lo Martire, V (author)
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Silvani, A (author)
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Swoap, SJ (author)
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Valli, A (author)
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Zoccoli, G (author)
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Cohen, G (author)
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- 2017-01-31
- 2017
- English.
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In: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7, s. 41698-
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https://www.nature.c...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
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- A major limitation in the study of sleep breathing disorders in mouse models of pathology is the need to combine whole-body plethysmography (WBP) to measure respiration with electroencephalography/electromyography (EEG/EMG) to discriminate wake-sleep states. However, murine wake-sleep states may be discriminated from breathing and body movements registered by the WBP signal alone. Our goal was to compare the EEG/EMG-based and the WBP-based scoring of wake-sleep states of mice, and provide formal guidelines for the latter. EEG, EMG, blood pressure and WBP signals were simultaneously recorded from 20 mice. Wake-sleep states were scored based either on EEG/EMG or on WBP signals and sleep-dependent respiratory and cardiovascular estimates were calculated. We found that the overall agreement between the 2 methods was 90%, with a high Cohen’s Kappa index (0.82). The inter-rater agreement between 2 experts and between 1 expert and 1 naïve sleep investigators gave similar results. Sleep-dependent respiratory and cardiovascular estimates did not depend on the scoring method. We show that non-invasive discrimination of the wake-sleep states of mice based on visual inspection of the WBP signal is accurate, reliable and reproducible. This work may set the stage for non-invasive high-throughput experiments evaluating sleep and breathing patterns on mouse models of pathophysiology.
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