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Luciferase Expressi...
Luciferase Expression Allows Bioluminescence Imaging But Imposes Limitations on the Orthotopic Mouse (4T1) Model of Breast Cancer
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Baklaushev, VP (author)
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Kilpelainen, A (author)
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- Petkov, S (author)
- Karolinska Institutet
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Abakumov, MA (author)
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Grinenko, NF (author)
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Yusubalieva, GM (author)
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Latanova, AA (author)
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Gubskiy, IL (author)
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Zabozlaev, FG (author)
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Starodubova, ES (author)
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Abakumova, TO (author)
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- Isaguliants, MG (author)
- Karolinska Institutet
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Chekhonin, VP (author)
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(creator_code:org_t)
- 2017-08-10
- 2017
- English.
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In: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7:1, s. 7715-
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https://www.nature.c...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
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- Implantation of reporter-labeled tumor cells in an immunocompetent host involves a risk of their immune elimination. We have studied this effect in a mouse model of breast cancer after the orthotopic implantation of mammary gland adenocarcinoma 4T1 cells genetically labelled with luciferase (Luc). Mice were implanted with 4T1 cells and two derivative Luc-expressing clones 4T1luc2 and 4T1luc2D6 exhibiting equal in vitro growth rates. In vivo, the daughter 4T1luc2 clone exhibited nearly the same, and 4T1luc2D6, a lower growth rate than the parental cells. The metastatic potential of 4T1 variants was assessed by magnetic resonance, bioluminescent imaging, micro-computed tomography, and densitometry which detected 100-μm metastases in multiple organs and bones at the early stage of their development. After 3–4 weeks, 4T1 generated 11.4 ± 2.1, 4T1luc2D6, 4.5 ± 0.6; and 4T1luc2, <1 metastases per mouse, locations restricted to lungs and regional lymph nodes. Mice bearing Luc-expressing tumors developed IFN-γ response to the dominant CTL epitope of Luc. Induced by intradermal DNA-immunization, such response protected mice from the establishment of 4T1luc2-tumors. Our data show that natural or induced cellular response against the reporter restricts growth and metastatic activity of the reporter-labelled tumor cells. Such cells represent a powerful instrument for improving immunization technique for cancer vaccine applications.
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- art (subject category)
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- By the author/editor
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Baklaushev, VP
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Kilpelainen, A
-
Petkov, S
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Abakumov, MA
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Grinenko, NF
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Yusubalieva, GM
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show more...
-
Latanova, AA
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Gubskiy, IL
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Zabozlaev, FG
-
Starodubova, ES
-
Abakumova, TO
-
Isaguliants, MG
-
Chekhonin, VP
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show less...
- Articles in the publication
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Scientific repor ...
- By the university
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Karolinska Institutet