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- Blom, S (author)
Systems pathology by multiplexed immunohistochemistry and whole-slide digital image analysis
- Article/chapterEnglish2017
Publisher, publication year, extent ...
- 2017-11-14
- Springer Science and Business Media LLC,2017
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- LIBRIS-ID:oai:prod.swepub.kib.ki.se:137049724
- http://kipublications.ki.se/Default.aspx?queryparsed=id:137049724URI
- https://doi.org/10.1038/s41598-017-15798-4DOI
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- Language:English
- Summary in:English
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- The paradigm of molecular histopathology is shifting from a single-marker immunohistochemistry towards multiplexed detection of markers to better understand the complex pathological processes. However, there are no systems allowing multiplexed IHC (mIHC) with high-resolution whole-slide tissue imaging and analysis, yet providing feasible throughput for routine use. We present an mIHC platform combining fluorescent and chromogenic staining with automated whole-slide imaging and integrated whole-slide image analysis, enabling simultaneous detection of six protein markers and nuclei, and automatic quantification and classification of hundreds of thousands of cells in situ in formalin-fixed paraffin-embedded tissues. In the first proof-of-concept, we detected immune cells at cell-level resolution (n = 128,894 cells) in human prostate cancer, and analysed T cell subpopulations in different tumour compartments (epithelium vs. stroma). In the second proof-of-concept, we demonstrated an automatic classification of epithelial cell populations (n = 83,558) and glands (benign vs. cancer) in prostate cancer with simultaneous analysis of androgen receptor (AR) and alpha-methylacyl-CoA (AMACR) expression at cell-level resolution. We conclude that the open-source combination of 8-plex mIHC detection, whole-slide image acquisition and analysis provides a robust tool allowing quantitative, spatially resolved whole-slide tissue cytometry directly in formalin-fixed human tumour tissues for improved characterization of histology and the tumour microenvironment.
Added entries (persons, corporate bodies, meetings, titles ...)
- Paavolainen, L (author)
- Bychkov, D (author)
- Turkki, R (author)
- Maki-Teeri, P (author)
- Hemmes, A (author)
- Valimaki, K (author)
- Lundin, JKarolinska Institutet (author)
- Kallioniemi, OKarolinska Institutet (author)
- Pellinen, T (author)
- Karolinska Institutet (creator_code:org_t)
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- In:Scientific reports: Springer Science and Business Media LLC7:1, s. 15580-2045-2322
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