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Altered Marginal Zo...
Altered Marginal Zone B Cell Selection in the Absence of IκBNS
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- Adori, M (author)
- Karolinska Institutet
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Pedersen, GK (author)
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Adori, C (author)
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- Erikson, E (author)
- Karolinska Institutet
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- Khoenkhoen, S (author)
- Karolinska Institutet
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- Stark, JM (author)
- Karolinska Institutet
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Choi, JH (author)
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- Dosenovic, P (author)
- Karolinska Institutet
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- Karlsson, MCI (author)
- Karolinska Institutet
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Beutler, B (author)
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- Hedestam, GBK (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2018-01-15
- 2018
- English.
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In: Journal of immunology (Baltimore, Md. : 1950). - : The American Association of Immunologists. - 1550-6606 .- 0022-1767. ; 200:2, s. 775-787
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https://doi.org/10.4...
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Abstract
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- Marginal zone (MZ) B cells reside in the splenic MZ and play important roles in T cell–independent humoral immune responses against blood-borne pathogens. IκBNS-deficient bumble mice exhibit a severe reduction in the MZ B compartment but regain an MZ B population with age and, thus, represent a valuable model to examine the biology of MZ B cells. In this article, we characterized the MZ B cell defect in further detail and investigated the nature of the B cells that appear in the MZ of aged bumble mice. Flow cytometry analysis of the splenic transitional B cell subsets demonstrated that MZ B cell development was blocked at the transitional-1 to transitional-2–MZ precursor stage in the absence of functional IκBNS. Immunohistochemical analysis of spleen sections from wild-type and bumble mice revealed no alteration in the cellular MZ microenvironment, and analysis of bone marrow chimeras indicated that the MZ B cell development defect in bumble mice was B cell intrinsic. Further, we demonstrate that the B cells that repopulate the MZ in aged bumble mice were distinct from age-matched wild-type MZ B cells. Specifically, the expression of surface markers characteristic for MZ B cells was altered and the L chain Igλ+ repertoire was reduced in bumble mice. Finally, plasma cell differentiation of sorted LPS-stimulated MZ B cells was impaired, and aged bumble mice were unable to respond to NP-Ficoll immunization. These results demonstrate that IκBNS is required for an intact MZ B cell compartment in C57BL/6 mice.
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- art (subject category)
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- By the author/editor
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Adori, M
-
Pedersen, GK
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Adori, C
-
Erikson, E
-
Khoenkhoen, S
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Stark, JM
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show more...
-
Choi, JH
-
Dosenovic, P
-
Karlsson, MCI
-
Beutler, B
-
Hedestam, GBK
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show less...
- Articles in the publication
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Journal of immun ...
- By the university
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Karolinska Institutet