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Sputum proteomics a...
Sputum proteomics and airway cell transcripts of current and ex-smokers with severe asthma in U-BIOPRED: an exploratory analysis
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Takahashi, K (author)
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Pavlidis, S (author)
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Kwong, FNK (author)
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Hoda, U (author)
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Rossios, C (author)
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Sun, K (author)
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Loza, M (author)
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Baribaud, F (author)
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Chanez, P (author)
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Fowler, SJ (author)
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Horvath, I (author)
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Montuschi, P (author)
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Singer, F (author)
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Musial, J (author)
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- Dahlen, B (author)
- Karolinska Institutet
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- Dahlen, SE (author)
- Karolinska Institutet
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Krug, N (author)
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- Sandström, Thomas, 1957- (author)
- Umeå universitet,Lungmedicin
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Shaw, DE (author)
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Lutter, R (author)
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Bakke, P (author)
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Fleming, LJ (author)
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Howarth, PH (author)
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Caruso, M (author)
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Sousa, AR (author)
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Corfield, J (author)
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Auffray, C (author)
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De Meulder, B (author)
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Lefaudeux, D (author)
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Djukanovic, R (author)
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Sterk, PJ (author)
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Guo, Y (author)
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Adcock, IM (author)
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Chung, KF (author)
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(creator_code:org_t)
- 2018-04-12
- 2018
- English.
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In: The European respiratory journal. - : European Respiratory Society (ERS). - 1399-3003 .- 0903-1936. ; 51:5
- Related links:
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https://erj.ersjourn...
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http://kipublication...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Severe asthma patients with a significant smoking history have airflow obstruction with reported neutrophilia. We hypothesise that multi-omic analysis will enable the definition of smoking and ex-smoking severe asthma molecular phenotypes.The U-BIOPRED cohort of severe asthma patients, containing current-smokers (CSA), ex-smokers (ESA), nonsmokers and healthy nonsmokers was examined. Blood and sputum cell counts, fractional exhaled nitric oxide and spirometry were obtained. Exploratory proteomic analysis of sputum supernatants and transcriptomic analysis of bronchial brushings, biopsies and sputum cells was performed.Colony-stimulating factor (CSF)2 protein levels were increased in CSA sputum supernatants, with azurocidin 1, neutrophil elastase and CXCL8 upregulated in ESA. Phagocytosis and innate immune pathways were associated with neutrophilic inflammation in ESA. Gene set variation analysis of bronchial epithelial cell transcriptome from CSA showed enrichment of xenobiotic metabolism, oxidative stress and endoplasmic reticulum stress compared to other groups. CXCL5 and matrix metallopeptidase 12 genes were upregulated in ESA and the epithelial protective genes, mucin 2 and cystatin SN, were downregulated.Despite little difference in clinical characteristics, CSA were distinguishable from ESA subjects at the sputum proteomic level, with CSA patients having increased CSF2 expression and ESA patients showing sustained loss of epithelial barrier processes.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Lungmedicin och allergi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Respiratory Medicine and Allergy (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Takahashi, K
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Pavlidis, S
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Kwong, FNK
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Hoda, U
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Rossios, C
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Sun, K
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show more...
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Loza, M
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Baribaud, F
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Chanez, P
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Fowler, SJ
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Horvath, I
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Montuschi, P
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Singer, F
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Musial, J
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Dahlen, B
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Dahlen, SE
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Krug, N
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Sandström, Thoma ...
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Shaw, DE
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Lutter, R
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Bakke, P
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Fleming, LJ
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Howarth, PH
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Caruso, M
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Sousa, AR
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Corfield, J
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Auffray, C
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De Meulder, B
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Lefaudeux, D
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Djukanovic, R
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Sterk, PJ
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Guo, Y
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Adcock, IM
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Chung, KF
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Respiratory Medi ...
- Articles in the publication
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The European res ...
- By the university
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Karolinska Institutet
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Umeå University