onr:"swepub:oai:prod.swepub.kib.ki.se:138739335"
Search: onr:"swepub:oai:prod.swepub.kib.ki.se:138739335" > Intravital imaging-...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Honkura, NaokiUppsala universitet,Vaskulärbiologi,Science for Life Laboratory, SciLifeLab (author)
Intravital imaging-based analysis tools for vessel identification and assessment of concurrent dynamic vascular events
- Article/chapterEnglish2018
Publisher, publication year, extent ...
- 2018-07-16
- Springer Science and Business Media LLC,2018
Numbers
- LIBRIS-ID:oai:prod.swepub.kib.ki.se:138739335
- http://kipublications.ki.se/Default.aspx?queryparsed=id:138739335URI
- https://doi.org/10.1038/s41467-018-04929-8DOI
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-361698URI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- The vasculature undergoes changes in diameter, permeability and blood flow in response to specific stimuli. The dynamics and interdependence of these responses in different vessels are largely unknown. Here we report a non-invasive technique to study dynamic events in different vessel categories by multi-photon microscopy and an image analysis tool, RVDM (relative velocity, direction, and morphology) allowing the identification of vessel categories by their red blood cell (RBC) parameters. Moreover, Claudin5 promoter-driven green fluorescent protein (GFP) expression is used to distinguish capillary subtypes. Intradermal injection of vascular endothelial growth factor A (VEGFA) is shown to induce leakage of circulating dextran, with vessel-type-dependent kinetics, from capillaries and venules devoid of GFP expression. VEGFA-induced leakage in capillaries coincides with vessel dilation and reduced flow velocity. Thus, intravital imaging of non-invasive stimulation combined with RVDM analysis allows for recording and quantification of very rapid events in the vasculature.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
- Richards, MarkUppsala universitet,Science for Life Laboratory, SciLifeLab,Vaskulärbiologi(Swepub:uu)marri386 (author)
- Laviña, BàrbaraUppsala universitet,Vaskulärbiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)barsi852 (author)
- Sainz-Jaspeado, MiguelUppsala universitet,Vaskulärbiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)migsa268 (author)
- Betsholtz, ChristerUppsala universitet,Karolinska Institutet,Vaskulärbiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)chbet517 (author)
- Claesson-Welsh, LenaUppsala universitet,Vaskulärbiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)lcl04207 (author)
- Uppsala universitetVaskulärbiologi (creator_code:org_t)
Related titles
- In:Nature communications: Springer Science and Business Media LLC9:1, s. 2746-2041-1723
Internet link
Find in a library
- Nature communications (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Honkura, Naoki
- Richards, Mark
- Laviña, Bàrbara
- Sainz-Jaspeado, ...
- Betsholtz, Chris ...
- Claesson-Welsh, ...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Cell and Molecul ...
- Articles in the publication
- Nature communica ...
- By the university
- Karolinska Institutet
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
