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Promoter Usage and ...
Promoter Usage and Dynamics in Vascular Smooth Muscle Cells Exposed to Fibroblast Growth Factor-2 or Interleukin-1β
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Alhendi, AMN (author)
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Patrikakis, M (author)
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- Daub, CO (author)
- Karolinska Institutet
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Kawaji, H (author)
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Itoh, M (author)
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de Hoon, M (author)
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Carninci, P (author)
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Hayashizaki, Y (author)
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Arner, E (author)
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Khachigian, LM (author)
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(creator_code:org_t)
- 2018-09-03
- 2018
- English.
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In: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 8:1, s. 13164-
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Abstract
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- Smooth muscle cells (SMC) in blood vessels are normally growth quiescent and transcriptionally inactive. Our objective was to understand promoter usage and dynamics in SMC acutely exposed to a prototypic growth factor or pro-inflammatory cytokine. Using cap analysis gene expression (FANTOM5 project) we report differences in promoter dynamics for immediate-early genes (IEG) and other genes when SMC are exposed to fibroblast growth factor-2 or interleukin-1β. Of the 1871 promoters responding to FGF2 or IL-1β considerably more responded to FGF2 (68.4%) than IL-1β (18.5%) and 13.2% responded to both. Expression clustering reveals sets of genes induced, repressed or unchanged. Among IEG responding rapidly to FGF2 or IL-1β were FOS, FOSB and EGR-1, which mediates human SMC migration. Motif activity response analysis (MARA) indicates most transcription factor binding motifs in response to FGF2 were associated with a sharp induction at 1 h, whereas in response to IL-1β, most motifs were associated with a biphasic change peaking generally later. MARA revealed motifs for FOS_FOS{B,L1}_JUN{B,D} and EGR-1..3 in the cluster peaking 1 h after FGF2 exposure whereas these motifs were in clusters peaking 1 h or later in response to IL-1β. Our findings interrogating CAGE data demonstrate important differences in promoter usage and dynamics in SMC exposed to FGF2 or IL-1β.
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- By the author/editor
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Alhendi, AMN
-
Patrikakis, M
-
Daub, CO
-
Kawaji, H
-
Itoh, M
-
de Hoon, M
-
show more...
-
Carninci, P
-
Hayashizaki, Y
-
Arner, E
-
Khachigian, LM
-
show less...
- Articles in the publication
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Scientific repor ...
- By the university
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Karolinska Institutet