High HMGB1 levels in sputum are related to pneumococcal bacteraemia but not to disease severity in community-acquired pneumonia

Alpkvist, HKarolinska Institutet,Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden; Unit of Infectious Diseases, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden (författare)

During bacterial infections, damage-associated molecular patterns (DAMPs) and pathogen-associated molecular patterns (PAMPs) activate immune cells. Here, we investigated whether plasma and sputum levels of High Mobility Group Box 1 (HMGB1), a prototypic DAMP, are associated with disease severity and aetiology in community-acquired pneumonia (CAP). In addition, in patients with pneumococcal CAP, the impact of the level of sputum lytA DNA load, a PAMP, was investigated. We studied patients hospitalised for bacterial CAP (n = 111), and samples were collected at admission. HMGB1 was determined by enzyme-linked immunosorbent assays, and pneumococcal lytA DNA load was determined by quantitative polymerase chain reaction. Plasma and sputum HMGB1 levels did not correlate to disease severity (pneumonia severity index or presence of sepsis), but high sputum HMGB1 level was correlated to pneumococcal aetiology (p = 0.002). In pneumococcal pneumonia, high sputum lytA DNA load was associated with respiratory failure (low PaO2/FiO2 ratio; p = 0.019), and high sputum HMGB1 level was associated with bacteraemia (p = 0.006). To conclude, high sputum HMGB1 was not associated with severe disease, but with pneumococcal bacteraemia, indicating a potential role for HMGB1 in bacterial dissemination. High sputum lytA was associated with severe disease.

Athlin, Simon,1971-Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Infectious Diseases(Swepub:oru)soan (författare)
Molling, PDepartment of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden (författare)
Norrby-Teglund, AKarolinska Institutet,Center for Infectious Medicine, Karolinska Institutet, Stockholm, Sweden (författare)
Stralin, KKarolinska Institutet,Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden; Unit of Infectious Diseases, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden (författare)
Karolinska InstitutetDepartment of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden; Unit of Infectious Diseases, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden (creator_code:org_t)

Ingår i:Scientific reports: Springer Science and Business Media LLC8:1, s. 13428-2045-2322

Av författaren/redakt...: Alpkvist, H; Athlin, Simon, 1 ...; Molling, P; Norrby-Teglund, ...; Stralin, K

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