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Differential B-Cell...
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Sadeghi, LKarolinska Institutet
(author)
Differential B-Cell Receptor Signaling Requirement for Adhesion of Mantle Cell Lymphoma Cells to Stromal Cells
- Article/chapterEnglish2020
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Numbers
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:143894730
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http://kipublications.ki.se/Default.aspx?queryparsed=id:143894730URI
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https://doi.org/10.3390/cancers12051143DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Interactions between lymphoma cells and stromal cells play a key role in promoting tumor survival and development of drug resistance. We identified differences in key signaling pathways between the JeKo-1 and REC-1 mantle cell lymphoma (MCL) cell lines, displaying different patterns of stromal cell adhesion and chemotaxis towards stroma-conditioned medium. The identified adhesion-regulated genes reciprocated important aspects of microenvironment-mediated gene modulation in MCL patients. Five-hundred and ninety genes were differently regulated between the cell lines upon adhesion to stromal cells, while 32 genes were similarly regulated in both cell lines. Regulation of B-cell Receptor (BCR) signature genes in adherent cells was specific for JeKo-1. Inhibition of BCR using siRNA or clinically approved inhibitors, Ibrutinib and Acalabrutinib, decreased adhesion of JeKo-1, but not REC-1 cells. Cell surface levels of chemokine receptor CXCR4 were higher in JeKo-1, facilitating migration and adhesion of JeKo-1 but not REC-1 cells. Surface levels of ICAM1 adhesion protein differ for REC-1 and JeKo-1. While ICAM1 played a positive role in adherence of both cell lines to stromal cells, S1PR1 had an inhibitory effect. Our results provide a model framework for further investigation of mechanistic differences in patient-response to new pathway-specific drugs.
Added entries (persons, corporate bodies, meetings, titles ...)
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Arvidsson, G
(author)
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Merrien, MKarolinska Institutet
(author)
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Wasik, AMKarolinska Institutet
(author)
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Gorgens, AKarolinska Institutet
(author)
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Smith, CIEKarolinska Institutet
(author)
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Sander, BKarolinska Institutet
(author)
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Wright, APKarolinska Institutet
(author)
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Karolinska Institutet
(creator_code:org_t)
Related titles
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In:Cancers: MDPI AG12:52072-6694
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