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Targeting Plk1 with...
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Goroshchuk, OKarolinska Institutet
(author)
Targeting Plk1 with siRNNs in primary cells from pediatric B-cell acute lymphoblastic leukemia patients
- Article/chapterEnglish2020
Publisher, publication year, extent ...
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2020-02-14
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Springer Science and Business Media LLC,2020
Numbers
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:144469021
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http://kipublications.ki.se/Default.aspx?queryparsed=id:144469021URI
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https://doi.org/10.1038/s41598-020-59653-5DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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B-cell acute lymphoblastic leukemia (B-ALL) accounts for nearly one fifth of all childhood cancers and current challenges in B-ALL treatment include resistance, relapse and late-onset side effects due to the chemotherapy. To overcome these hurdles, novel therapies need to be investigated. One promising target is Polo-like kinase 1 (Plk1), a key regulator of the cell cycle. In this study, the Plk family expression is investigated in primary peripheral blood and bone marrow mononuclear cells from ten pediatric B-ALL patients. For the first time, short interfering RiboNucleic Neutrals (siRNNs) that enter cells without a transfection reagent are used to target Plk1 mRNA in primary cells from pediatric B-ALL patients. Our results show that the expression of Plk1 and Plk4 is significantly higher in pediatric B-ALL patients compared to healthy donors. Moreover, treatment of primary peripheral blood and bone marrow mononuclear cells from pediatric B-ALL patients, cultured ex vivo, with Plk1-targeting siRNNs results in cleavage of Plk1 mRNA. Importantly, the Plk1 knockdown is specific and does not affect other Plk members in contrast to many small molecule Plk1 inhibitors. Thus, Plk1 is a potential therapeutic target in pediatric B-ALL and selective targeting of Plk1 can be achieved by the use of siRNNs.
Added entries (persons, corporate bodies, meetings, titles ...)
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Vidarsdottir, L
(author)
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Bjorklund, ACKarolinska Institutet
(author)
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Hamil, AS
(author)
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Kolosenko, IKarolinska Institutet
(author)
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Dowdy, SF
(author)
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Palm-Apergi, CKarolinska Institutet
(author)
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Karolinska Institutet
(creator_code:org_t)
Related titles
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In:Scientific reports: Springer Science and Business Media LLC10:1, s. 2688-2045-2322
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