onr:"swepub:oai:prod.swepub.kib.ki.se:144744856"
Search: onr:"swepub:oai:prod.swepub.kib.ki.se:144744856" > Massive and rapid C...
- 1 of 1
- Previous record
- Next record
- To hitlist
Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
-
- Smyrlaki, I (author)
- Karolinska Institutet
- Ekman, M (author)
-
- Lentini, A (author)
- Karolinska Institutet
- show more...
- Vondracek, M (author)
- Aarum, J (author)
- Safari, H (author)
- Muradrasoli, S (author)
- show less...
- (creator_code:org_t)
- 2020-09-23
- 2020
- English.
- In: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 11:1, s. 4812-
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but RNA extraction constitutes a major bottleneck in current testing. Methodological simplification could increase diagnostic availability and efficiency, benefitting patient care and infection control. Here, we describe methods circumventing RNA extraction in COVID-19 testing by performing RT-PCR directly on heat-inactivated or lysed samples. Our data, including benchmarking using 597 clinical patient samples and a standardised diagnostic system, demonstrate that direct RT-PCR is viable option to extraction-based tests. Using controlled amounts of active SARS-CoV-2, we confirm effectiveness of heat inactivation by plaque assay and evaluate various generic buffers as transport medium for direct RT-PCR. Significant savings in time and cost are achieved through RNA-extraction-free protocols that are directly compatible with established PCR-based testing pipelines. This could aid expansion of COVID-19 testing.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Nature communications (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Smyrlaki, I
- Ekman, M
- Lentini, A
- de Sousa, NR
- Papanicolaou, N
- Vondracek, M
- show more...
- Aarum, J
- Safari, H
- Muradrasoli, S
- Rothfuchs, AG
- Albert, J
- Hogberg, B
- Reinius, B
- show less...
- Articles in the publication
- Nature communica ...
- By the university
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
