onr:"swepub:oai:prod.swepub.kib.ki.se:145193750"
Search: onr:"swepub:oai:prod.swepub.kib.ki.se:145193750" > Selection, biophysi...
- 1 of 1
- Previous record
- Next record
- To hitlist
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2
- Custodio, TF (author)
-
- Das, H (author)
- Karolinska Institutet
-
- Sheward, DJ (author)
- Karolinska Institutet
- show more...
- Pazicky, S (author)
- Pieprzyk, J (author)
- Sorgenfrei, M (author)
- Schroer, MA (author)
- Gruzinov, AY (author)
- Jeffries, CM (author)
- Graewert, MA (author)
- Svergun, DI (author)
- Dobrev, N (author)
- Remans, K (author)
- Seeger, MA (author)
- Low, C (author)
- show less...
- (creator_code:org_t)
- 2020-11-04
- 2020
- English.
- In: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 11:1, s. 5588-
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- The coronavirus SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Therapeutic neutralizing antibodies constitute a key short-to-medium term approach to tackle COVID-19. However, traditional antibody production is hampered by long development times and costly production. Here, we report the rapid isolation and characterization of nanobodies from a synthetic library, known as sybodies (Sb), that target the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. Several binders with low nanomolar affinities and efficient neutralization activity were identified of which Sb23 displayed high affinity and neutralized pseudovirus with an IC50 of 0.6 µg/ml. A cryo-EM structure of the spike bound to Sb23 showed that Sb23 binds competitively in the ACE2 binding site. Furthermore, the cryo-EM reconstruction revealed an unusual conformation of the spike where two RBDs are in the ‘up’ ACE2-binding conformation. The combined approach represents an alternative, fast workflow to select binders with neutralizing activity against newly emerging viruses.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Nature communications (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Custodio, TF
- Das, H
- Sheward, DJ
- Hanke, L
- Pazicky, S
- Pieprzyk, J
- show more...
- Sorgenfrei, M
- Schroer, MA
- Gruzinov, AY
- Jeffries, CM
- Graewert, MA
- Svergun, DI
- Dobrev, N
- Remans, K
- Seeger, MA
- McInerney, GM
- Murrell, B
- Hallberg, BM
- Low, C
- show less...
- Articles in the publication
- Nature communica ...
- By the university
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
