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Comparison of Cytom...
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Wagner-Drouet, E
(author)
Comparison of Cytomegalovirus-Specific Immune Cell Response to Proteins versus Peptides Using an IFN-γ ELISpot Assay after Hematopoietic Stem Cell Transplantation
- Article/chapterEnglish2021
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:145951544
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http://kipublications.ki.se/Default.aspx?queryparsed=id:145951544URI
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https://doi.org/10.3390/diagnostics11020312DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic stem cell transplantation (HSCT). Measuring CMV-specific cellular immunity may improve the risk stratification and management of patients. IFN-γ ELISpot assays, based on the stimulation of peripheral blood mononuclear cells with CMV pp65 and IE-1 proteins or peptides, have been validated in clinical settings. However, it remains unclear to which extend the T-cell response to synthetic peptides reflect that mediated by full-length proteins processed by antigen-presenting cells. We compared the stimulating ability of pp65 and IE-1 proteins and corresponding overlapping peptides in 16 HSCT recipients using a standardized IFN-γ ELISpot assay. Paired qualitative test results showed an overall 74.4% concordance. Discordant results were mainly due to low-response tests, with one exception. One patient with early CMV reactivation and graft-versus-host disease, sustained CMV DNAemia and high CD8+ counts showed successive negative protein-based ELISpot results but a high and sustained response to IE-1 peptides. Our results suggest that the response to exogenous proteins, which involves their uptake and processing by antigen-presenting cells, more closely reflects the physiological response to CMV infection, while the response to exogenous peptides may lead to artificial in vitro T-cell responses, especially in strongly immunosuppressed patients.
Added entries (persons, corporate bodies, meetings, titles ...)
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Teschner, D
(author)
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Wolschke, C
(author)
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Schafer-Eckart, K
(author)
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Gartner, J
(author)
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Mielke, SKarolinska Institutet
(author)
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Schreder, M
(author)
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Kobbe, G
(author)
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Hilgendorf, I
(author)
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Klein, S
(author)
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Verbeek, M
(author)
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Ditschkowski, M
(author)
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Koch, M
(author)
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Lindemann, M
(author)
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Schmidt, T
(author)
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Rascle, A
(author)
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Barabas, S
(author)
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Deml, L
(author)
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Wagner, R
(author)
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Wolff, D
(author)
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Karolinska Institutet
(creator_code:org_t)
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In:Diagnostics (Basel, Switzerland): MDPI AG11:22075-4418
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Wagner-Drouet, E
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Teschner, D
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Wolschke, C
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Schafer-Eckart, ...
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Gartner, J
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Mielke, S
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Schreder, M
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Kobbe, G
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Hilgendorf, I
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Klein, S
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Verbeek, M
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Ditschkowski, M
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Koch, M
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Lindemann, M
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Schmidt, T
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Rascle, A
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Barabas, S
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Deml, L
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Wagner, R
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Wolff, D
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Diagnostics (Bas ...
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Karolinska Institutet