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Somatic mutations i...
Somatic mutations in lymphocytes in patients with immune-mediated aplastic anemia
- Article/chapterEnglish2021
Publisher, publication year, extent ...
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2021-03-30
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Springer Science and Business Media LLC,2021
Numbers
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:146281247
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http://kipublications.ki.se/Default.aspx?queryparsed=id:146281247URI
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https://doi.org/10.1038/s41375-021-01231-3DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The prevalence and functional impact of somatic mutations in nonleukemic T cells is not well characterized, although clonal T-cell expansions are common. In immune-mediated aplastic anemia (AA), cytotoxic T-cell expansions are shown to participate in disease pathogenesis. We investigated the mutation profiles of T cells in AA by a custom panel of 2533 genes. We sequenced CD4+ and CD8+ T cells of 24 AA patients and compared the results to 20 healthy controls and whole-exome sequencing of 37 patients with AA. Somatic variants were common both in patients and healthy controls but enriched to AA patients’ CD8+ T cells, which accumulated most mutations on JAK-STAT and MAPK pathways. Mutation burden was associated with CD8+ T-cell clonality, assessed by T-cell receptor beta sequencing. To understand the effect of mutations, we performed single-cell sequencing of AA patients carrying STAT3 or other mutations in CD8+ T cells. STAT3 mutated clone was cytotoxic, clearly distinguishable from other CD8+ T cells, and attenuated by successful immunosuppressive treatment. Our results suggest that somatic mutations in T cells are common, associate with clonality, and can alter T-cell phenotype, warranting further investigation of their role in the pathogenesis of AA.
Added entries (persons, corporate bodies, meetings, titles ...)
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Keranen, MAI
(author)
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Kankainen, M
(author)
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Huuhtanen, J
(author)
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Walldin, G
(author)
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Kerr, CM
(author)
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Clemente, M
(author)
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Ebeling, F
(author)
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Rajala, H
(author)
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Bruck, O
(author)
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Lahdesmaki, H
(author)
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Hannula, S
(author)
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Hannunen, T
(author)
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Ellonen, P
(author)
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Young, NS
(author)
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Ogawa, S
(author)
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Maciejewski, JP
(author)
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Hellstrom-Lindberg, EKarolinska Institutet
(author)
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Mustjoki, S
(author)
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Karolinska Institutet
(creator_code:org_t)
Related titles
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In:Leukemia: Springer Science and Business Media LLC35:75, s. 1365-13791476-55510887-6924
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Leukemia
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Lundgren, S
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Keranen, MAI
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Kankainen, M
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Huuhtanen, J
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Walldin, G
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Kerr, CM
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show more...
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Clemente, M
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Ebeling, F
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Rajala, H
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Bruck, O
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Lahdesmaki, H
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Hannula, S
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Hannunen, T
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Ellonen, P
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Young, NS
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Ogawa, S
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Maciejewski, JP
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Hellstrom-Lindbe ...
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Mustjoki, S
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Leukemia
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Karolinska Institutet