onr:"swepub:oai:prod.swepub.kib.ki.se:146693920"
Search: onr:"swepub:oai:prod.swepub.kib.ki.se:146693920" > Short-term IL-15 pr...
- 1 of 1
- Previous record
- Next record
- To hitlist
Short-term IL-15 priming leaves a long-lasting signalling imprint in mouse NK cells independently of a metabolic switch
- Luu, TT (author)
- Schmied, L (author)
- Nguyen, NA (author)
- show more...
- Wiel, C (author)
- Ganesan, S (author)
- show less...
- (creator_code:org_t)
- 2021-02-16
- 2021
- English.
- In: Life science alliance. - : Life Science Alliance, LLC. - 2575-1077. ; 4:4
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- IL-15 priming of NK cells is a broadly accepted concept, but the dynamics and underlying molecular mechanisms remain poorly understood. We show that as little as 5 min of IL-15 treatment in vitro, followed by removal of excess cytokines, results in a long-lasting, but reversible, augmentation of NK cell responsiveness upon activating receptor cross-linking. In contrast to long-term stimulation, improved NK cell function after short-term IL-15 priming was not associated with enhanced metabolism but was based on the increased steady-state phosphorylation level of signalling molecules downstream of activating receptors. Inhibition of JAK3 eliminated this priming effect, suggesting a cross talk between the IL-15 receptor and ITAM-dependent activating receptors. Increased signalling molecule phosphorylation levels, calcium flux, and IFN-γ secretion lasted for up to 3 h after IL-15 stimulation before returning to baseline. We conclude that IL-15 rapidly and reversibly primes NK cell function by modulating activating receptor signalling. Our findings suggest a mechanism by which NK cell reactivity can potentially be maintained in vivo based on only brief encounters with IL-15 trans-presenting cells.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Life science alliance (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Luu, TT
- Schmied, L
- Nguyen, NA
- Wiel, C
- Meinke, S
- Mohammad, DK
- show more...
- Bergo, M
- Alici, E
- Kadri, N
- Ganesan, S
- Hoglund, P
- show less...
- Articles in the publication
- Life science all ...
- By the university
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
