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Human Cytomegalovir...
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Yaiw, KCKarolinska Institutet
(author)
Human Cytomegalovirus Reduces Endothelin-1 Expression in Both Endothelial and Vascular Smooth Muscle Cells
- Article/chapterEnglish2021
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:146977080
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http://kipublications.ki.se/Default.aspx?queryparsed=id:146977080URI
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https://doi.org/10.3390/microorganisms9061137DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Human cytomegalovirus (HCMV) is an opportunistic pathogen that has been implicated in the pathogenesis of atherosclerosis. Endothelin-1 (ET-1), a potent vasoconstrictive peptide, is overexpressed and strongly associated with many vasculopathies. The main objective of this study was to investigate whether HCMV could affect ET-1 production. As such, both endothelial and smooth muscle cells, two primary cell types involved in the pathogenesis of atherosclerosis, were infected with HCMV in vitro and ET-1 mRNA and proteins were assessed by quantitative PCR assay, immunofluorescence staining and ELISA. HCMV infection significantly decreased ET-1 mRNA and secreted bioactive ET-1 levels from both cell types and promoted accumulation of the ET-1 precursor protein in infected endothelial cells. This was associated with inhibition of expression of the endothelin converting enzyme-1 (ECE-1), which cleaves the ET-1 precursor protein to mature ET-1. Ganciclovir treatment did not prevent the virus suppressive effects on ET-1 expression. Consistent with this observation we identified that the IE2-p86 protein predominantly modulated ET-1 expression. Whether the pronounced effects of HCMV in reducing ET-1 expression in vitro may lead to consequences for regulation of the vascular tone in vivo remains to be proven.
Added entries (persons, corporate bodies, meetings, titles ...)
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Mohammad, AA
(author)
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Taher, C
(author)
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Cui, HL
(author)
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Costa, H
(author)
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Kostopoulou, ONKarolinska Institutet
(author)
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Jung, M
(author)
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Assinger, A
(author)
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Wilhelmi, V
(author)
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Yang, JNKarolinska Institutet
(author)
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Straat, K
(author)
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Rahbar, AKarolinska Institutet
(author)
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Pernow, JKarolinska Institutet
(author)
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Soderberg-Naucler, CKarolinska Institutet
(author)
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Karolinska Institutet
(creator_code:org_t)
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In:Microorganisms: MDPI AG9:62076-2607
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Yaiw, KC
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Mohammad, AA
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Taher, C
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Cui, HL
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Costa, H
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Kostopoulou, ON
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Jung, M
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Assinger, A
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Wilhelmi, V
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Yang, JN
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Straat, K
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Rahbar, A
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Pernow, J
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Soderberg-Naucle ...
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Microorganisms
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Karolinska Institutet