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Amnion signals are ...
Amnion signals are essential for mesoderm formation in primates
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- Yang, R (author)
- Karolinska Institutet
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- Goedel, A (author)
- Karolinska Institutet
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Kang, Y (author)
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Si, CY (author)
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Chu, C (author)
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Zheng, Y (author)
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Chen, ZZ (author)
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Gruber, PJ (author)
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- Xiao, Y (author)
- Karolinska Institutet
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Zhou, CK (author)
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- Witman, N (author)
- Karolinska Institutet
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Leung, CY (author)
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Chen, YC (author)
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Fu, JP (author)
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Ji, WZ (author)
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- Lanner, F (author)
- Karolinska Institutet
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Niu, YY (author)
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- Chien, K (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2021-08-26
- 2021
- English.
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In: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 12:1, s. 5126-
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Abstract
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- Embryonic development is largely conserved among mammals. However, certain genes show divergent functions. By generating a transcriptional atlas containing >30,000 cells from post-implantation non-human primate embryos, we uncover that ISL1, a gene with a well-established role in cardiogenesis, controls a gene regulatory network in primate amnion. CRISPR/Cas9-targeting of ISL1 results in non-human primate embryos which do not yield viable offspring, demonstrating that ISL1 is critically required in primate embryogenesis. On a cellular level, mutant ISL1 embryos display a failure in mesoderm formation due to reduced BMP4 signaling from the amnion. Via loss of function and rescue studies in human embryonic stem cells we confirm a similar role of ISL1 in human in vitro derived amnion. This study highlights the importance of the amnion as a signaling center during primate mesoderm formation and demonstrates the potential of in vitro primate model systems to dissect the genetics of early human embryonic development.
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- art (subject category)
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- By the author/editor
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Yang, R
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Goedel, A
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Kang, Y
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Si, CY
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Chu, C
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Zheng, Y
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show more...
-
Chen, ZZ
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Gruber, PJ
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Xiao, Y
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Zhou, CK
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Witman, N
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Leung, CY
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Chen, YC
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Fu, JP
-
Ji, WZ
-
Lanner, F
-
Niu, YY
-
Chien, K
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show less...
- Articles in the publication
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Nature communica ...
- By the university
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Karolinska Institutet