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Deep characterizati...
Deep characterization of paired chromatin and transcriptomes in four immune cell types from multiple sclerosis patients
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Fernandes, SJ (author)
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Ericsson, M (author)
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- Khademi, M (author)
- Karolinska Institutet
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- Jagodic, M (author)
- Karolinska Institutet
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- Olsson, T (author)
- Karolinska Institutet
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Gomez-Cabrero, D (author)
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- Kockum, I (author)
- Karolinska Institutet
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Tegner, J (author)
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(creator_code:org_t)
- Future Medicine Ltd, 2021
- 2021
- English.
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In: Epigenomics. - : Future Medicine Ltd. - 1750-192X .- 1750-1911. ; 13:20, s. 1607-1618
- Related links:
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http://kipublication...
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https://doi.org/10.2...
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Abstract
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- Background: The putative involvement of chromatin states in multiple sclerosis (MS) is thus far unclear. Here we determined the association of chromatin-accessibility with concurrent genetic, epigenetic and transcriptional events. Material & methods: We generated paired assay for transposase-accessible chromatin sequencing and RNA-sequencing profiles from sorted blood immune CD4+ and CD8+ T cells, CD14+ monocytes and CD19+ B cells from healthy controls (HCs) and MS patients. Results: We identified differentially accessible regions between MS patients and HCs, primarily in CD4+ and CD19+. CD4+ regions were enriched for MS-associated single nucleotide polymorphisms and differentially methylated loci. In the vicinity of differentially accessible regions of CD4+ cells, 42 differentially expressed genes were identified. The top two dysregulated genes identified in this multilayer analysis were CCDC114 and SERTAD1. Conclusion: These findings provide new insight into the primary role of CD4+ and CD19+ cells in MS.
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