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Profiling of ob/ob ...
Profiling of ob/ob mice skeletal muscle exosome-like vesicles demonstrates combined action of miRNAs, proteins and lipids to modulate lipid homeostasis in recipient cells
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Jalabert, A (author)
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Reininger, L (author)
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Berger, E (author)
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Coute, Y (author)
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Meugnier, E (author)
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Forterre, A (author)
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Errazuriz-Cerda, E (author)
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Geloen, A (author)
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Aouadi, M (author)
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Bouzakri, K (author)
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Rieusset, J (author)
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Rome, S (author)
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- 2021-11-03
- 2021
- English.
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In: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 11:1, s. 21626-
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https://www.nature.c...
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https://doi.org/10.1...
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Abstract
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- We have determined the lipid, protein and miRNA composition of skeletal muscle (SkM)-released extracellular vesicles (ELVs) from Ob/ob (OB) vs wild-type (WT) mice. The results showed that atrophic insulin-resistant OB-SkM released less ELVs than WT-SkM, highlighted by a RAB35 decrease and an increase in intramuscular cholesterol content. Proteomic analyses of OB-ELVs revealed a group of 37 proteins functionally connected, involved in lipid oxidation and with catalytic activities. OB-ELVs had modified contents for phosphatidylcholine (PC 34-4, PC 40-3 and PC 34-0), sphingomyelin (Sm d18:1/18:1) and ceramides (Cer d18:1/18:0) and were enriched in cholesterol, likely to alleviated intracellular accumulation. Surprisingly many ELV miRNAs had a nuclear addressing sequence, and targeted genes encoding proteins with nuclear activities. Interestingly, SkM-ELV miRNA did not target mitochondria. The most significant function targeted by the 7 miRNAs altered in OB-ELVs was lipid metabolism. In agreement, OB-ELVs induced lipid storage in recipient adipocytes and increased lipid up-take and fatty acid oxidation in recipient muscle cells. In addition, OB-ELVs altered insulin-sensitivity and induced atrophy in muscle cells, reproducing the phenotype of the releasing OB muscles. These data suggest for the first time, a cross-talk between muscle cells and adipocytes, through the SkM-ELV route, in favor of adipose tissue expansion.
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- By the author/editor
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Jalabert, A
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Reininger, L
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Berger, E
-
Coute, Y
-
Meugnier, E
-
Forterre, A
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show more...
-
Errazuriz-Cerda, ...
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Geloen, A
-
Aouadi, M
-
Bouzakri, K
-
Rieusset, J
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Rome, S
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show less...
- Articles in the publication
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Scientific repor ...
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Karolinska Institutet