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Prime-seq, efficien...
Prime-seq, efficient and powerful bulk RNA sequencing
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Janjic, A (author)
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Wange, LE (author)
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Bagnoli, JW (author)
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Geuder, J (author)
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Nguyen, P (author)
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Richter, D (author)
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Vieth, B (author)
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Vick, B (author)
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Jeremias, I (author)
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- Ziegenhain, C (author)
- Karolinska Institutet
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Hellmann, I (author)
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Enard, W (author)
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(creator_code:org_t)
- 2022-03-31
- 2022
- English.
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In: Genome biology. - : Springer Science and Business Media LLC. - 1474-760X. ; 23:1, s. 88-
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https://doi.org/10.1...
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Abstract
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- Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq, a standard bulk RNA-seq method, but is fourfold more cost-efficient due to almost 50-fold cheaper library costs. We also validate a direct RNA isolation step, show that intronic reads are derived from RNA, and compare cost-efficiencies of available protocols. We conclude that prime-seq is currently one of the best options to set up an early barcoding bulk RNA-seq protocol from which many labs would profit.
Subject headings
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
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- art (subject category)
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- By the author/editor
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Janjic, A
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Wange, LE
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Bagnoli, JW
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Geuder, J
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Nguyen, P
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Richter, D
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show more...
-
Vieth, B
-
Vick, B
-
Jeremias, I
-
Ziegenhain, C
-
Hellmann, I
-
Enard, W
-
show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Bioinformatics a ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Medical Genetics
- Articles in the publication
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Genome biology
- By the university
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Karolinska Institutet