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  • Raivola, J (author)

Multiomics characterization implicates PTK7 in ovarian cancer EMT and cell plasticity and offers strategies for therapeutic intervention

  • Article/chapterEnglish2022

Publisher, publication year, extent ...

  • 2022-08-17
  • Springer Science and Business Media LLC,2022

Numbers

  • LIBRIS-ID:oai:prod.swepub.kib.ki.se:150466527
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:150466527URI
  • https://doi.org/10.1038/s41419-022-05161-5DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Most patients with ovarian cancer (OC) are diagnosed at a late stage when there are very few therapeutic options and a poor prognosis. This is due to the lack of clearly defined underlying mechanisms or an oncogenic addiction that can be targeted pharmacologically, unlike other types of cancer. Here, we identified protein tyrosine kinase 7 (PTK7) as a potential new therapeutic target in OC following a multiomics approach using genetic and pharmacological interventions. We performed proteomics analyses upon PTK7 knockdown in OC cells and identified novel downstream effectors such as synuclein-γ (SNCG), SALL2, and PP1γ, and these findings were corroborated in ex vivo primary samples using PTK7 monoclonal antibody cofetuzumab. Our phosphoproteomics analyses demonstrated that PTK7 modulates cell adhesion and Rho-GTPase signaling to sustain epithelial-mesenchymal transition (EMT) and cell plasticity, which was confirmed by high-content image analysis of 3D models. Furthermore, using high-throughput drug sensitivity testing (525 drugs) we show that targeting PTK7 exhibited synergistic activity with chemotherapeutic agent paclitaxel, CHK1/2 inhibitor prexasertib, and PLK1 inhibitor GSK461364, among others, in OC cells and ex vivo primary samples. Taken together, our study provides unique insight into the function of PTK7, which helps to define its role in mediating aberrant Wnt signaling in ovarian cancer.

Added entries (persons, corporate bodies, meetings, titles ...)

  • Dini, A (author)
  • Karvonen, H (author)
  • Piki, E (author)
  • Salokas, K (author)
  • Niininen, W (author)
  • Kaleva, L (author)
  • Zhang, KY (author)
  • Arjama, M (author)
  • Gudoityte, G (author)
  • Seashore-Ludlow, BKarolinska Institutet (author)
  • Varjosalo, M (author)
  • Kallioniemi, OKarolinska Institutet (author)
  • Hautaniemi, S (author)
  • Murumagi, A (author)
  • Ungureanu, D (author)
  • Karolinska Institutet (creator_code:org_t)

Related titles

  • In:Cell death & disease: Springer Science and Business Media LLC13:8, s. 714-2041-4889

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