Search: onr:"swepub:oai:prod.swepub.kib.ki.se:150483425" >
Chemokine Receptor ...
Chemokine Receptor Expression on T Cells Is Modulated by CAFs and Chemokines Affect the Spatial Distribution of T Cells in Pancreatic Tumors
-
- Gorchs, L (author)
- Karolinska Institutet
-
Oosthoek, M (author)
-
- Yucel-Lindberg, T (author)
- Karolinska Institutet
-
show more...
-
- Moro, CF (author)
- Karolinska Institutet
-
- Kaipe, H (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- 2022-08-06
- 2022
- English.
-
In: Cancers. - : MDPI AG. - 2072-6694. ; 14:15
- Related links:
-
http://kipublication...
-
show more...
-
https://doi.org/10.3...
-
show less...
Abstract
Subject headings
Close
- The accumulation of T cells is associated with a better prognosis in pancreatic cancer. However, the immunosuppressive tumor microenvironment, largely composed by cancer-associated fibroblasts (CAFs), can prevent T cells from reaching the tumor nests. We examined how human CAFs modulated chemokine receptors known to be associated with T cell trafficking, CXCR3 and CCR5, and T cell exclusion, CXCR4. CAFs decreased the expression of CXCR3 and CCR5 but increased CXCR4 expression in both 2D and 3D cultures, affecting the migratory capacity of T cells towards CXCL10. An immunohistochemistry analysis showed that very few T cells were found in the tumor nests. Within the stroma, CD8+ T cells were localized more distantly from the malignant cells whereas CD4+ T cells were more equally distributed. Tumor tissues with a high production of chemokines were associated with less T cell infiltration when the whole tissue was analyzed. However, when the spatial localization of CD8+ T cells within the tissue was taken into account, levels of CXCR3 ligands and the CCR5 ligand CCL8 showed a positive association with a high relative T cell infiltration in tumor-rich areas. Thus, CXCR3 ligands could mediate T cell trafficking but CAFs could prevent T cells from reaching the malignant cells.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
Cancers
(Search for host publication in LIBRIS)
To the university's database