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A competitive preci...
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites
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Pihlajamaa, P (author)
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Kauko, O (author)
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Sahu, B (author)
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Kivioja, T (author)
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- Taipale, J (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2022-09-26
- 2023
- English.
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In: Nature biotechnology. - : Springer Science and Business Media LLC. - 1546-1696 .- 1087-0156. ; 41:32, s. 197-
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https://doi.org/10.1...
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Abstract
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- Here we describe a competitive genome editing method that measures the effect of mutations on molecular functions, based on precision CRISPR editing using template libraries with either the original or altered sequence, and a sequence tag, enabling direct comparison between original and mutated cells. Using the example of the MYC oncogene, we identify important transcriptional targets and show that E-box mutations at MYC target gene promoters reduce cellular fitness.
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- art (subject category)
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