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Subinhibitory antib...
Subinhibitory antibiotic concentrations promote the horizontal transfer of plasmid-borne resistance genes from Klebsiellae pneumoniae to Escherichia coli
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Ding, ML (author)
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Ye, Z (author)
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Liu, L (author)
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Wang, W (author)
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Chen, Q (author)
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Zhang, FY (author)
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Wang, Y (author)
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- Sjoling, A (author)
- Karolinska Institutet
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- Martin-Rodriguez, AJ (author)
- Karolinska Institutet
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Hu, RJ (author)
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Chen, WB (author)
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Zhou, YS (author)
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(creator_code:org_t)
- 2022-11-07
- 2022
- English.
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In: Frontiers in microbiology. - : Frontiers Media SA. - 1664-302X. ; 13, s. 1017092-
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https://doi.org/10.3...
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Abstract
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- Horizontal gene transfer plays an important role in the spread of antibiotic resistance, in which plasmid-mediated conjugation transfer is the most important mechanism. While sub-minimal inhibitory concentrations (sub-MIC) of antibiotics could promote conjugation frequency, the mechanism by which sub-MIC levels of antibiotics affect conjugation frequency is not clear. Here, we used Klebsiella pneumoniae SW1780 carrying the multi-drug resistance plasmid pSW1780-KPC as the donor strain, to investigate the effects of sub-MICs of meropenem (MEM), ciprofloxacin (CIP), cefotaxime (CTX), and amikacin (AK) on conjugational transfer of pSW1780-KPC from SW1780 to Escherichia coli J53. Our results showed that the transfer frequencies increased significantly by treating SW1780 strain with sub-MIC levels of MEM, CIP, CTX and AK. Transfer frequencies at sub-MIC conditions in a Galleria mellonella were significantly higher than in vitro. To investigate gene expression and metabolic effects, RT-qPCR and LC–MS-based metabolome sequencing were performed. Transcript levels of T4SS genes virB1, virB2, virB4, virB8, and conjugation-related genes traB, traK, traE, and traL were significantly upregulated by exposure to sub-MICs of MEM, CIP, CTX, and AK. Metabolome sequencing revealed nine differentially regulated metabolites. Our findings are an early warning for a wide assessment of the roles of sub-MIC levels of antibiotics in the spread of antibiotic resistance.
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- By the author/editor
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Ding, ML
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Ye, Z
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Liu, L
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Wang, W
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Chen, Q
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Zhang, FY
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show more...
-
Wang, Y
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Sjoling, A
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Martin-Rodriguez ...
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Hu, RJ
-
Chen, WB
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Zhou, YS
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show less...
- Articles in the publication
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Frontiers in mic ...
- By the university
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Karolinska Institutet