onr:"swepub:oai:prod.swepub.kib.ki.se:151647724"
Search: onr:"swepub:oai:prod.swepub.kib.ki.se:151647724" > Target-enriched nan...
- 1 of 1
- Previous record
- Next record
- To hitlist
Target-enriched nanopore sequencing and de novo assembly reveals co-occurrences of complex on-target genomic rearrangements induced by CRISPR-Cas9 in human cells
-
- Geng, KY (author)
- Karolinska Institutet
-
- Merino, LG (author)
- Karolinska Institutet
- Wedemann, L (author)
- show more...
- Martens, A (author)
- Sobota, M (author)
- Sondergaard, JN (author)
- White, RJ (author)
- show less...
- (creator_code:org_t)
- 2022-09-30
- 2022
- English.
- In: Genome research. - : Cold Spring Harbor Laboratory. - 1549-5469 .- 1088-9051. ; 32:10, s. 1876-1891
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- The CRISPR-Cas9 system is widely used to permanently delete genomic regions via dual guide RNAs. Genomic rearrangements induced by CRISPR-Cas9 can occur, but continuous technical developments make it possible to characterize complex on-target effects. We combined an innovative droplet-based target enrichment approach with long-read sequencing and coupled it to a customized de novo sequence assembly. This approach enabled us to dissect the sequence content at kilobase scale within an on-target genomic locus. We here describe extensive genomic disruptions by Cas9, involving the allelic co-occurrence of a genomic duplication and inversion of the target region, as well as integrations of exogenous DNA and clustered interchromosomal DNA fragment rearrangements. Furthermore, we found that these genomic alterations led to functional aberrant DNA fragments and can alter cell proliferation. Our findings broaden the consequential spectrum of the Cas9 deletion system, reinforce the necessity of meticulous genomic validations, and introduce a data-driven workflow enabling detailed dissection of the on-target sequence content with superior resolution.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Genome research (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Geng, KY
- Merino, LG
- Wedemann, L
- Martens, A
- Sobota, M
- Sanchez, YP
- show more...
- Sondergaard, JN
- White, RJ
- Kutter, C
- show less...
- Articles in the publication
- Genome research
- By the university
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
