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VEGF-A splice varia...
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Gustafsson, TKarolinska Institutet
(author)
VEGF-A splice variants and related receptor expression in human skeletal muscle following submaximal exercise
- Article/chapterEnglish2005
Publisher, publication year, extent ...
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American Physiological Society,2005
Numbers
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:1950802
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1950802URI
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https://doi.org/10.1152/japplphysiol.01402.2004DOI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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VEGF-A contributes to muscle tissue angiogenesis following aerobic exercise training. The temporal response of the VEGF-A isoforms and their target receptors has not been comprehensively profiled in human skeletal muscle. We combined submaximal exercise with and without reduced leg blood flow to establish whether ischemia-induced metabolic stress was an important physiological stimuli responsible for regulating the VEGF-A system in humans. Nine healthy men performed two 45-min bouts of one-leg knee-extension exercise, with and without blood flow restriction. Muscle biopsies were obtained at rest and 2 and 6 h after exercise. Expression (mRNA) of the VEGF-A splice variants and related receptors [VEGF receptor (VEGFR)-1, VEGFR-2, and neuropilin-1] was determined by using qPCR. VEGF-Atotal expression increased more robustly after exercise with reduced blood flow, and initially this principally reflected an increase in VEGF-A165. Six hours after exercise, there was a relatively greater increase in VEGF-A189, and this response was not influenced by blood flow conditions. VEGFR-1 mRNA expression increased 2 h after exercise, and neuropilin-1 expression was transiently reduced, while all three receptors increased by 6 h. There was no evidence for the expression of the inhibitory VEGF-A165B variant in human skeletal muscle. Our study, reflecting both VEGF-A ligand and receptors, implicates metabolic perturbation as a regulator of human muscle angiogenesis and demonstrates that VEGF-A splice variants are distinctly regulated. Our findings also indicate that all three receptor genes exhibit different pretranslational regulation, in response to exercise in humans.
Added entries (persons, corporate bodies, meetings, titles ...)
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Ameln, HKarolinska Institutet
(author)
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Fischer, HKarolinska Institutet
(author)
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Sundberg, CJKarolinska Institutet
(author)
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Timmons, JA
(author)
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Jansson, EKarolinska Institutet
(author)
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Karolinska Institutet
(creator_code:org_t)
Related titles
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In:Journal of applied physiology (Bethesda, Md. : 1985): American Physiological Society98:6, s. 2137-21468750-75871522-1601
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