Dissimilar Deformation of Fluid- and Gel-Phase Liposomes upon Multivalent Interaction with Cell Membrane Mimics Revealed Using Dual-Wavelength Surface Plasmon Resonance

Norling, Karin,1988Chalmers tekniska högskola,Chalmers University of Technology,Division of Nano and Biophysics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden (author)

The mechanical properties of biological nanoparticles play a crucial role in their interaction with the cellular membrane, in particular for cellular uptake. This has significant implications for the design of pharmaceutical carrier particles. In this context, liposomes have become increasingly popular, among other reasons due to their customizability and easily varied physicochemical properties. With currently available methods, it is, however, not trivial to characterize the mechanical properties of nanoscopic liposomes especially with respect to the level of deformation induced upon their ligand-receptor-mediated interaction with laterally fluid cellular membranes. Here, we utilize the sensitivity of dual-wavelength surface plasmon resonance to probe the size and shape of bound liposomes (∼100 nm in diameter) as a means to quantify receptor-induced deformation during their interaction with a supported cell membrane mimic. By comparing biotinylated liposomes in gel and fluid phases, we demonstrate that fluid-phase liposomes are more prone to deformation than their gel-phase counterparts upon binding to the cell membrane mimic and that, as expected, the degree of deformation depends on the number of ligand-receptor pairs that are engaged in the multivalent binding.

Sjöberg, Mattias,1991Chalmers tekniska högskola,Chalmers University of Technology,Division of Nano and Biophysics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden(Swepub:cth)matsjob (author)
Bally, Marta,1981Umeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Umeå University(Swepub:umu)maba0261 (author)
Zhdanov, Vladimir,1952Russian Academy of Sciences,Chalmers tekniska högskola,Chalmers University of Technology,Division of Nano and Biophysics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden; Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk, Russian Federation(Swepub:cth)zhdanov (author)
Parveen, Nagma,1988Chalmers tekniska högskola,Chalmers University of Technology,Division of Nano and Biophysics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden(Swepub:cth)nagma (author)
Höök, Fredrik,1966Chalmers tekniska högskola,Chalmers University of Technology,Division of Nano and Biophysics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden(Swepub:cth)fredrikh (author)
Chalmers tekniska högskolaDivision of Nano and Biophysics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden (creator_code:org_t)

In:Langmuir: American Chemical Society (ACS)38:8, s. 2550-25601520-58270743-7463

By the author/editor: Norling, Karin, ...; Sjöberg, Mattias ...; Bally, Marta, 19 ...; Zhdanov, Vladimi ...; Parveen, Nagma, ...; Höök, Fredrik, 1 ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Other Basic Medi ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biophysics

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Physical Chemist ...

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