Search: onr:"swepub:oai:research.chalmers.se:18768a21-4be0-4cac-b3c0-33a34952c0d4" >
Lipid vesicle compo...
Lipid vesicle composition influences the incorporation and fluorescence properties of the lipophilic sulphonated carbocyanine dye SP-DiO
-
- Lubart, Quentin, 1989 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Hannestad, Jonas, 1981 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Pace, Hudson, 1982 (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Umeå University
-
show more...
-
- Fjällborg, Daniel, 1994 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Westerlund, Fredrik, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Esbjörner Winters, Elin, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Bally, Marta, 1981 (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Umeå University
-
Esbjörner, Elin K. (author)
-
show less...
-
(creator_code:org_t)
- 2020
- 2020
- English.
-
In: Physical Chemistry Chemical Physics. - : Royal Society of Chemistry (RSC). - 1463-9084 .- 1463-9076. ; 22:16, s. 8781-8790
- Related links:
-
https://research.cha... (primary) (free)
-
show more...
-
https://pubs.rsc.org...
-
https://doi.org/10.1...
-
https://umu.diva-por... (primary) (Raw object)
-
https://doi.org/10.1...
-
https://research.cha...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Lipophilic carbocyanine dyes are widely used as fluorescent cell membrane probes in studies ranging from biophysics to cell biology. While they are extremely useful for qualitative observation of lipid structures, a major problem impairing quantitative studies is that the chemical environment of the lipid bilayer affects both the dye's insertion efficiency and photophysical properties. We present a systematic investigation of the sulphonated carbocyanine dye 3,3′-dioctadecyl-5,5′-di(4-sulfophenyl) (SP-DiO) and demonstrate how its insertion efficiency into pre-formed lipid bilayers and its photophysical properties therein determine its apparent fluorescence intensity in different lipid environments. For this purpose, we use large unilamellar vesicles (LUVs) made of lipids with distinct chain unsaturation, acyl chain length, head group charge, and with variation in membrane cholesterol content as models. Using a combination of absorbance, fluorescence emission, and fluorescence lifetime measurements we reveal that SP-DiO incorporates more efficiently into liquid disordered phases compared to gel phases. Moreover, incorporation into the latter phase is most efficient when the mismatch between the length of the lipid and dye hydrocarbon chains is small. Furthermore, SP-DiO incorporation is less efficient in LUVs composed of negatively charged lipids. Lastly, when cholesterol was included in the LUV membranes, we observed significant spectral shifts, consistent with dye aggregation. Taken together, our study highlights the complex interplay between membrane composition and labeling efficiency with lipophilic dyes and advocates for careful assessment of fluorescence data when attempting a quantitative analysis of fluorescence data with such molecules.
Subject headings
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi -- Annan kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Other Chemistry Topics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Lubart, Quentin, ...
-
Hannestad, Jonas ...
-
Pace, Hudson, 19 ...
-
Fjällborg, Danie ...
-
Westerlund, Fred ...
-
Esbjörner Winter ...
-
show more...
-
Bally, Marta, 19 ...
-
Esbjörner, Elin ...
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Chemical Science ...
-
and Physical Chemist ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Chemical Science ...
-
and Other Chemistry ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biophysics
- Articles in the publication
-
Physical Chemist ...
- By the university
-
Chalmers University of Technology
-
Umeå University