Search: onr:"swepub:oai:research.chalmers.se:2cb0c415-3d6d-41a9-98dd-f856534ec0db" >
Autologous endothel...
Autologous endothelialisation by the stromal vascular fraction on laminin-bioconjugated nanocellulose-alginate scaffolds
-
- Oskarsdotter, Kristin, 1995 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery
-
- Säljö, Karin, 1981 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery
-
- Sämfors, Sanna, 1987 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
show more...
-
- Niemi, Essi M. (author)
- Universitetet i Oslo,University of Oslo,Oslo universitetssykehus,Oslo University Hospital
-
- Li, Susann (author)
- Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital
-
- Simonsson, Stina, 1969 (author)
- Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital
-
- Apelgren, Peter (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery
-
- Scholz, Hanne (author)
- Universitetet i Oslo,University of Oslo
-
- Gatenholm, Paul, 1956 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Kölby, Lars, 1963 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery
-
show less...
-
(creator_code:org_t)
- 2023
- 2023
- English.
-
In: Biomedical Materials (Bristol). - 1748-605X .- 1748-6041. ; 18:4
- Related links:
-
https://research.cha... (primary) (free)
-
show more...
-
https://research.cha...
-
https://doi.org/10.1...
-
https://gup.ub.gu.se...
-
show less...
Abstract
Subject headings
Close
- Establishing a vascular network in biofabricated tissue grafts is essential for ensuring graft survival. Such networks are dependent on the ability of the scaffold material to facilitate endothelial cell adhesion; however, the clinical translation potential of tissue-engineered scaffolds is hindered by the lack of available autologous sources of vascular cells. Here, we present a novel approach to achieving autologous endothelialisation in nanocellulose-based scaffolds by using adipose tissue-derived vascular cells on nanocellulose-based scaffolds. We used sodium periodate-mediated bioconjugation to covalently bind laminin to the scaffold surface and isolated the stromal vascular fraction and endothelial progenitor cells (EPCs; CD31+CD45−) from human lipoaspirate. Additionally, we assessed the adhesive capacity of scaffold bioconjugation in vitro using both adipose tissue-derived cell populations and human umbilical vein endothelial cells. The results showed that the bioconjugated scaffold exhibited remarkably higher cell viability and scaffold surface coverage by adhesion regardless of cell type, whereas control groups comprising cells on non-bioconjugated scaffolds exhibited minimal cell adhesion across all cell types. Furthermore, on culture day 3, EPCs seeded on laminin-bioconjugated scaffolds showed positive immunofluorescence staining for the endothelial markers CD31 and CD34, suggesting that the scaffolds promoted progenitor differentiation into mature endothelial cells. These findings present a possible strategy for generating autologous vasculature and thereby increase the clinical relevance of 3D-bioprinted nanocellulose-based constructs.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk material- och protesteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Materials (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Keyword
- autologous endothelialisation
- adipose tissue
- stromal vascular fraction
- nanocellulose-alginate hydrogels
- protein bioconjugation
- sodium periodate oxidation
- autologous endothelialisation
- nanocellulose-alginate hydrogels
- adipose
- tissue
- sodium periodate oxidation
- protein bioconjugation
- stromal vascular fraction
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Oskarsdotter, Kr ...
-
Säljö, Karin, 19 ...
-
Sämfors, Sanna, ...
-
Niemi, Essi M.
-
Li, Susann
-
Simonsson, Stina ...
-
show more...
-
Apelgren, Peter
-
Scholz, Hanne
-
Gatenholm, Paul, ...
-
Kölby, Lars, 196 ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
-
- ENGINEERING AND TECHNOLOGY
-
ENGINEERING AND ...
-
and Medical Engineer ...
-
and Medical Material ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Cancer and Oncol ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Medical Biotechn ...
-
and Biomaterials Sci ...
- Articles in the publication
-
Biomedical Mater ...
- By the university
-
Chalmers University of Technology
-
University of Gothenburg