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Production of 10-me...
Production of 10-methyl branched fatty acids in yeast
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Blitzblau, Hannah G. (author)
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Consiglio, Andrew L. (author)
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- Teixeira, Paulo, 1990 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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Crabtree, Donald V. (author)
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Chen, Shuyan (author)
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- Konzock, Oliver, 1991 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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Chifamba, Gamuchirai (author)
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Su, Austin (author)
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Kamineni, Annapurna (author)
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MacEwen, Kyle (author)
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Hamilton, Maureen (author)
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Tsakraklides, Vasiliki (author)
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- Nielsen, Jens B, 1962 (author)
- BioInnovation Institute (BII),Chalmers tekniska högskola,Chalmers University of Technology,Novo Nordisk Fonden,Novo Nordisk Foundation
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- Siewers, Verena, 1976 (author)
- Novo Nordisk Fonden,Novo Nordisk Foundation,Chalmers tekniska högskola,Chalmers University of Technology
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Shaw, A. Joe (author)
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(creator_code:org_t)
- 2021-01-07
- 2021
- English.
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In: Biotechnology for Biofuels. - : Springer Science and Business Media LLC. - 1754-6834 .- 1754-6834. ; 14:1
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https://biotechnolog...
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Abstract
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- Background: Despite the environmental value of biobased lubricants, they account for less than 2% of global lubricant use due to poor thermo-oxidative stability arising from the presence of unsaturated double bonds. Methyl branched fatty acids (BFAs), particularly those with branching near the acyl-chain mid-point, are a high-performance alternative to existing vegetable oils because of their low melting temperature and full saturation. Results: We cloned and characterized two pathways to produce 10-methyl BFAs isolated from actinomycetes and γ-proteobacteria. In the two-step bfa pathway of actinomycetes, BfaB methylates Δ9 unsaturated fatty acids to form 10-methylene BFAs, and subsequently, BfaA reduces the double bond to produce a fully saturated 10-methyl branched fatty acid. A BfaA-B fusion enzyme increased the conversion efficiency of 10-methyl BFAs. The ten-methyl palmitate production (tmp) pathway of γ-proteobacteria produces a 10-methylene intermediate, but the TmpA putative reductase was not active in E. coli or yeast. Comparison of BfaB and TmpB activities revealed a range of substrate specificities from C14-C20 fatty acids unsaturated at the Δ9, Δ10 or Δ11 position. We demonstrated efficient production of 10-methylene and 10-methyl BFAs in S. cerevisiae by secretion of free fatty acids and in Y. lipolytica as triacylglycerides, which accumulated to levels more than 35% of total cellular fatty acids. Conclusions: We report here the characterization of a set of enzymes that can produce position-specific methylene and methyl branched fatty acids. Yeast expression of bfa enzymes can provide a platform for the large-scale production of branched fatty acids suitable for industrial and consumer applications.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Keyword
- 10-Methylstearic acid
- Tuberculostearic acid
- Yarrowia lipolytica
- Biobased lubricant
Publication and Content Type
- art (subject category)
- ref (subject category)
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Blitzblau, Hanna ...
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Consiglio, Andre ...
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Teixeira, Paulo, ...
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Crabtree, Donald ...
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Chen, Shuyan
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Konzock, Oliver, ...
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show more...
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Chifamba, Gamuch ...
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Su, Austin
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Kamineni, Annapu ...
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MacEwen, Kyle
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Hamilton, Mauree ...
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Tsakraklides, Va ...
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Nielsen, Jens B, ...
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Siewers, Verena, ...
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Shaw, A. Joe
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show less...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
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and Biocatalysis and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Organic Chemistr ...
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Biotechnology fo ...
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Chalmers University of Technology