onr:"swepub:oai:research.chalmers.se:35d85f71-3e08-474b-93c6-bc5afc6ab82d"
Search: onr:"swepub:oai:research.chalmers.se:35d85f71-3e08-474b-93c6-bc5afc6ab82d" > HMF and furfural st...
- 1 of 1
- Previous record
- Next record
- To hitlist
HMF and furfural stress results in drainage of redox and energy charge of Saccharomyces cerevisiae
-
- Ask, Magnus, 1983 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Bettiga, Maurizio, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Mapelli, Valeria, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
- show more...
- show less...
- (creator_code:org_t)
- 2012
- 2012
- English.
- In: 13th International Congress on Yeasts, Madison, WI, USA.
- Conference paper (other academic/artistic)
Abstract
Subject headings
Close
- Bioethanol produced from lignocellulosic raw materials is a promising alternative to fossil fuels and to decrease greenhouse gas emissions, but several challenges still exist. When lignocellulosic biomass is pretreated, a number of undesired degradation products are generated which may act inhibitory on microbial metabolism. Cellular damage response and repair come at an energy cost for the cell, which could be reflected by alterations in (energy) metabolism. The furaldehydes HMF and furfural have received increasing attention recently. They are formed during pretreatment from dehydration of hexoses and pentoses, respectively. In the present study, the effects of HMF and furfural on redox metabolism, energy metabolism and transcriptome were investigated. Anaerobic chemostat cultivations were performed with the xylose-utilizing Saccharomyces cerevisiae strain VTT C-10883 with both glucose and xylose as carbon sources. By quantifying the redox cofactors NAD(P)+ and NAD(P)H, the catabolic and anabolic reduction charges could be calculated. It was found that both reduction charges were significantly decreased in the presence of HMF and furfural, showing that HMF and furfural are draining the cells of reductive power. Furthermore, the [ATP]/[ADP] ratio of stressed cells was found to be lower than for non-stressed cells, suggesting that the energy metabolism was affected. Transcriptome analysis revealed that genes involved in xenobiotic transporter activity were significantly enriched among the up-regulated genes. The results from the present study provide valuable insights of how Saccharomyces cerevisiae deals with stress imposed by HMF and furfural, which potentially can result in strategies to improve stress tolerance.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioenergi (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioenergy (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Publication and Content Type
- kon (subject category)
- vet (subject category)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Ask, Magnus, 198 ...
- Bettiga, Maurizi ...
- Mapelli, Valeria ...
- Olsson, Lisbeth, ...
- About the subject
-
- ENGINEERING AND TECHNOLOGY
- ENGINEERING AND ...
- and Industrial Biote ...
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Biochemistry and ...
-
- ENGINEERING AND TECHNOLOGY
- ENGINEERING AND ...
- and Industrial Biote ...
- and Bioenergy
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Bioinformatics a ...
- Articles in the publication
- 13th Internation ...
- By the university
- Chalmers University of Technology
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
