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Self-assembly forma...
Self-assembly formation of multiple DNA-tethered lipid bilayers
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- Tabaei, Seyed, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Jönsson, Peter, 1981 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Brändén, Magnus, 1971 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Höök, Fredrik, 1966 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- Elsevier BV, 2009
- 2009
- English.
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In: Journal of Structural Biology. - : Elsevier BV. - 1047-8477 .- 1095-8657. ; 168:1, s. 200-206
- Related links:
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http://dx.doi.org/10...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Inspired by natural cell-cell junctions, where membrane-residing proteins control the separation between two or more membranes without interfering with their integrity, we report a new self-assembly route for formation of multiple highly fluid tethered lipid bilayers with the inter-membrane volume geometrically confined by membrane-anchored DNA duplexes. The formation of multiple planar membrane-membrane junctions were accomplished using disk shaped bicelles, composed of a mixture of the long-chained dimyristoyl phosphatidylcholine (DMPC) and the short-chained dihexanoyl PC further stabilized with the positively charged detergent hexadecyl-trimethyl-ammonium bromide (CTAB). Quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) were used to monitor the formation and to characterize the integrity of the self-assembled lipid-DNA architecture. (C) 2009 Elsevier Inc. All rights reserved.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Annan teknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Other Engineering and Technologies (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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