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Pneumatic stimulati...
Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap
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- Nekimken, A. L. (author)
- Stanford University
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- Fehlauer, H. (author)
- Stanford University
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- Kim, Anna, 1989 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Manosalvas-Kjono, S. N. (author)
- Stanford University
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- Ladpli, P. (author)
- Stanford University
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- Memon, F. (author)
- Stanford University
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- Gopisetty, D. (author)
- Stanford University
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- Sanchez, V. (author)
- Stanford University
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- Goodman, M. B. (author)
- Stanford University
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- Pruitt, B. L. (author)
- Stanford University
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- Krieg, M. (author)
- Stanford University
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(creator_code:org_t)
- 2017
- 2017
- English.
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In: Lab on a Chip - Miniaturisation for Chemistry and Biology. - : Royal Society of Chemistry (RSC). - 1473-0189 .- 1473-0197. ; 17:6, s. 1116-1127
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Abstract
Subject headings
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- New tools for applying force to animals, tissues, and cells are critically needed in order to advance the field of mechanobiology, as few existing tools enable simultaneous imaging of tissue and cell deformation as well as cellular activity in live animals. Here, we introduce a novel microfluidic device that enables high-resolution optical imaging of cellular deformations and activity while applying precise mechanical stimuli to the surface of the worm's cuticle with a pneumatic pressure reservoir. To evaluate device performance, we compared analytical and numerical simulations conducted during the design process to empirical measurements made with fabricated devices. Leveraging the well-characterized touch receptor neurons (TRNs) with an optogenetic calcium indicator as a model mechanoreceptor neuron, we established that individual neurons can be stimulated and that the device can effectively deliver steps as well as more complex stimulus patterns. This microfluidic device is therefore a valuable platform for investigating the mechanobiology of living animals and their mechanosensitive neurons.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Mechanosensory Neurons
- DEG/ENAC
- Ion Channels
- Touch Receptor Neurons
- On-Chip
- Light
- Mechanotransduction
- Sensation
- Differentiation
- Caenorhabditis-Elegans
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Nekimken, A. L.
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Fehlauer, H.
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Kim, Anna, 1989
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Manosalvas-Kjono ...
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Ladpli, P.
-
Memon, F.
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show more...
-
Gopisetty, D.
-
Sanchez, V.
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Goodman, M. B.
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Pruitt, B. L.
-
Krieg, M.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Lab on a Chip - ...
- By the university
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Chalmers University of Technology