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Visualization of β-...
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Brackmann, Christian,1973Chalmers tekniska högskola,Chalmers University of Technology
(author)
Visualization of β-carotene and starch granules in plant cells using CARS and SHG microscopy
- Article/chapterEnglish2010
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LIBRIS-ID:oai:research.chalmers.se:4a9a7680-6351-478d-b885-4e2aeb982473
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https://doi.org/10.1002/jrs.2778DOI
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https://research.chalmers.se/publication/136353URI
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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Information on the content and bioavailability of provitamin A carotenoids, such as beta-carotene, in plant foods is of great interest due to the widespread vitamin A deficiency in developing countries. While the amount of beta-carotene can readily be quantified with analytical techniques, there is limited information on beta-carotene morphology in native plant materials. Here, we introduce nonlinear microscopy for three-dimensional, label-free imaging of carotenoids in fresh and thermally treated plant tissues, providing quantitative information at single-aggregate level and detailed insight into their distribution. Carotenoids in orange-fleshed sweet potato (OFSP), carrot, and mango were visualized by coherent anti-Stokes Raman scattering (CARS) microscopy and, in the case of OFSP, related to the plant-matrix morphology by simultaneous second-harmonic generation (SHG) microscopy of starch granules. Sizes, shapes, densities, and location of different types of carotenoid bodies were quantified. While OFSP and carrot showed heterogeneous rod-shaped bodies with high carotenoid densities indicated by higher CARS signals, the carotenoid-filled lipid droplets in mango appeared as homogeneous low-density aggregates of rounded shape. In addition, beta-carotene densities and morphologies in OFSP were studied after thermal processing, showing that the bodies remain intact despite significant changes of the surrounding starch granules.
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Bengtsson, Anton,1979Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)kb99bean
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Alminger, Marie,1957Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)mariealm
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Svanberg, Ulf,1945Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)ulfsva
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Enejder, Annika,1969Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)enejder
(author)
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Chalmers tekniska högskola
(creator_code:org_t)
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In:Journal of Raman Spectroscopy: Wiley42:4, s. 586-5920377-04861097-4555
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