Search: onr:"swepub:oai:research.chalmers.se:50206968-c2ba-41cd-beb1-2fd2f71eaf12" >
Enabling large-scal...
Enabling large-scale genome editing at repetitive elements by reducing DNA nicking
-
- Smith, Cory J. (author)
- Harvard University
-
- Castanon, Oscar (author)
- École polytechnique,Harvard University
-
- Said, Khaled (author)
- Harvard Medical School
-
show more...
-
- Volf, Verena (author)
- Harvard University
-
- Khoshakhlagh, Parastoo (author)
- Harvard University
-
- Hornick, Amanda (author)
- Harvard University
-
- Ferreira, Raphael, 1990 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Wu, Chun Ting (author)
- Harvard Medical School
-
- Güell, Marc (author)
- Universitat Pompeu Fabra,University Pompeu Fabra
-
- Garg, Shilpa (author)
- Harvard Medical School
-
- Ng, Alex H.M. (author)
- Harvard University
-
- Myllykallio, Hannu (author)
- École polytechnique
-
- Church, George M. (author)
- Harvard Medical School
-
show less...
-
(creator_code:org_t)
- 2020-04-21
- 2020
- English.
-
In: Nucleic Acids Research. - : Oxford University Press (OUP). - 0305-1048 .- 1362-4962. ; 48:9, s. 5183-5195
- Related links:
-
https://research.cha... (primary) (free)
-
show more...
-
https://academic.oup...
-
https://doi.org/10.1...
-
https://research.cha...
-
show less...
Abstract
Subject headings
Close
- To extend the frontier of genome editing and enable editing of repetitive elements of mammalian genomes, we made use of a set of dead-Cas9 base editor (dBE) variants that allow editing at tens of thousands of loci per cell by overcoming the cell death associated with DNA double-strand breaks and single-strand breaks. We used a set of gRNAs targeting repetitive elements-ranging in target copy number from about 32 to 161 000 per cell. dBEs enabled survival after large-scale base editing, allowing targeted mutations at up to ∼13 200 and ∼12 200 loci in 293T and human induced pluripotent stem cells (hiPSCs), respectively, three orders of magnitude greater than previously recorded. These dBEs can overcome current on-target mutation and toxicity barriers that prevent cell survival after large-scale genome engineering.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Smith, Cory J.
-
Castanon, Oscar
-
Said, Khaled
-
Volf, Verena
-
Khoshakhlagh, Pa ...
-
Hornick, Amanda
-
show more...
-
Ferreira, Raphae ...
-
Wu, Chun Ting
-
Güell, Marc
-
Garg, Shilpa
-
Ng, Alex H.M.
-
Myllykallio, Han ...
-
Church, George M ...
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Cell Biology
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Medical Biotechn ...
-
and Medical Biotechn ...
- Articles in the publication
-
Nucleic Acids Re ...
- By the university
-
Chalmers University of Technology