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Kinetic and thermod...
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Gunnarsson, Anders,1981Chalmers tekniska högskola,Chalmers University of Technology
(author)
Kinetic and thermodynamic characterization of single-mismatch discrimination using single-molecule imaging
- Article/chapterEnglish2009
Publisher, publication year, extent ...
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2009-06-09
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Oxford University Press (OUP),2009
Numbers
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LIBRIS-ID:oai:research.chalmers.se:54ceb1d7-57c6-4526-81e2-f28ef71befbb
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https://research.chalmers.se/publication/115413URI
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https://doi.org/10.1093/nar/gkp487DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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A single-molecule detection setup based on total internal reflection fluorescence (TIRF) microscopy has been used to investigate association and dissociation kinetics of unlabeled 30mer DNA strands. Single-molecule sensitivity was accomplished by letting unlabeled DNA target strands mediate the binding of DNA-modified and fluorescently labeled liposomes to a DNA-modified surface. The liposomes, acting as signal enhancer elements, enabled the number of binding events as well as the residence time for high affinity binders (K-d
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Jönsson, Peter,1981Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)jopeter
(author)
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Zhdanov, Vladimir,1952Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)zhdanov
(author)
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Höök, Fredrik,1966Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)fredrikh
(author)
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Chalmers tekniska högskola
(creator_code:org_t)
Related titles
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In:Nucleic Acids Research: Oxford University Press (OUP)37:14, s. e99 (art no)-0305-10481362-4962
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