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Optimized in vitro ...
Optimized in vitro and in vivo expression of proteorhodopsin: A seven-transmembrane proton pump
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- Gourdon, Pontus Emanuel, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Alfredsson, Anna, 1982 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
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- Pedersen, Anders, 1976 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
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- Malmerberg, Erik, 1980 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
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- Nyblom, Anna Maria, 1975 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Widell, Mikael (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Berntsson, Ronnie (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Rijksuniversiteit Groningen,University of Groningen
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- Pinhassi, Jarone (author)
- Linnéuniversitetet,Linnaeus University
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- Braiman, Marc (author)
- Syracuse University
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- Hansson, Örjan, 1955 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
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- Bonander, Nicklas, 1968 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
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- Karlsson, B Göran, 1962 (author)
- Gothenburg University,Göteborgs universitet,Svenskt NMR-centrum vid Göteborgs universitet,Swedish NMR Centre at Göteborg University
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- Neutze, Richard, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
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(creator_code:org_t)
- Elsevier BV, 2008
- 2008
- English.
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In: Protein Expression and Purification. - : Elsevier BV. - 1096-0279 .- 1046-5928. ; 58:1, s. 103-113
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http://dx.doi.org/10...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Proteorhodopsin is an integral membrane light-harvesting proton pump that is found in bacteria distributed throughout global surface waters. Here, we present a protocol for functional in vitro production of pR using a commercial cell-free synthesis system yielding 1.0 mg purified protein per milliliter of cell lysate. We also present an optimized protocol for in vivo over-expression of pR in Escherichia coli, and a two-step purification yielding 5 mg of essentially pure functional protein per liter of culture. Both approaches are straightforward, rapid, and easily scalable. Thus either may facilitate the exploitation of pR for commercial biotechnological applications. Finally, the implications of some observations of the in vitro synthesis behavior, as well as preliminary results towards a structural determination of pR are discussed.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Membrane protein
- Functional expression
- Cell-free
- Proteorhodopsin
- Proteorhodopsin; Membrane protein; Cell-free; Functional expression
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Gourdon, Pontus ...
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Alfredsson, Anna ...
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Pedersen, Anders ...
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Malmerberg, Erik ...
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Nyblom, Anna Mar ...
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Widell, Mikael
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show more...
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Berntsson, Ronni ...
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Pinhassi, Jarone
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Braiman, Marc
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Hansson, Örjan, ...
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Bonander, Nickla ...
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Karlsson, B Göra ...
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Neutze, Richard, ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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Protein Expressi ...
- By the university
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Chalmers University of Technology
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University of Gothenburg