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Fluorescence recove...
Fluorescence recovery after photobleaching in material and life sciences: Putting theory into practice
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- Lorén, Niklas, 1970 (author)
- RISE,Structure Design
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- Hagman, Joel H, 1983 (author)
- RISE,SP – Sveriges Tekniska Forskningsinstitut
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- Jonasson, Jenny, 1976 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Göteborgs universitet,University of Gothenburg,Chalmers University of Technology, Sweden
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- Deschout, Hendrik (author)
- Universiteit Gent,Ghent university,Ghent University, Belgium
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- Bernin, Diana, 1979 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Göteborgs universitet,University of Gothenburg,Chalmers University of Technology, Sweden
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- Cella-Zanacchi, F. (author)
- Fondazione Istituto Italiano di Tecnologia,Istituto Italiano di Tecnologia, Italy
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- Diaspro, A. (author)
- Fondazione Istituto Italiano di Tecnologia,Istituto Italiano di Tecnologia, Italy
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- McNally, J.G. (author)
- Helmholtz Center Berlin, Germany
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- Ameloot, M. (author)
- Universiteit Hasselt,Hasselt University, Belgium
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- Smisdom, N. (author)
- Universiteit Hasselt,Vlaamse Instelling voor Technologisch Onderzoek (VITO),Flemish Institute for Technological Research,Hasselt University, Belgium
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- Nydén, Magnus, 1970 (author)
- University of South Australia,University of South Australia, Australia
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- Hermansson, Anne-Marie, 1945 (author)
- RISE,Structure Design
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- Rudemo, Mats, 1937 (author)
- Göteborgs universitet,University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden
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- Braeckmans, Kevin (author)
- Universiteit Gent,Ghent university,Ghent University, Belgium
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(creator_code:org_t)
- 2015
- 2015
- English.
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In: Quarterly Reviews of Biophysics. - 1469-8994 .- 0033-5835. ; 48:3, s. 323-387
- Related links:
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http://dx.doi.org/10...
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Abstract
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- Copyright © 2015 Cambridge University Press.Fluorescence recovery after photobleaching (FRAP) is a versatile tool for determining diffusion and interaction/binding properties in biological and material sciences. An understanding of the mechanisms controlling the diffusion requires a deep understanding of structure-interaction-diffusion relationships. In cell biology, for instance, this applies to the movement of proteins and lipids in the plasma membrane, cytoplasm and nucleus. In industrial applications related to pharmaceutics, foods, textiles, hygiene products and cosmetics, the diffusion of solutes and solvent molecules contributes strongly to the properties and functionality of the final product. All these systems are heterogeneous, and accurate quantification of the mass transport processes at the local level is therefore essential to the understanding of the properties of soft (bio)materials. FRAP is a commonly used fluorescence microscopy-based technique to determine local molecular transport at the micrometer scale. A brief high-intensity laser pulse is locally applied to the sample, causing substantial photobleaching of the fluorescent molecules within the illuminated area. This causes a local concentration gradient of fluorescent molecules, leading to diffusional influx of intact fluorophores from the local surroundings into the bleached area. Quantitative information on the molecular transport can be extracted from the time evolution of the fluorescence recovery in the bleached area using a suitable model. A multitude of FRAP models has been developed over the years, each based on specific assumptions. This makes it challenging for the non-specialist to decide which model is best suited for a particular application. Furthermore, there are many subtleties in performing accurate FRAP experiments. For these reasons, this review aims to provide an extensive tutorial covering the essential theoretical and practical aspects so as to enable accurate quantitative FRAP experiments for molecular transport measurements in soft (bio)materials.
Subject headings
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
- NATURVETENSKAP -- Matematik -- Annan matematik (hsv//swe)
- NATURAL SCIENCES -- Mathematics -- Other Mathematics (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Lorén, Niklas, 1 ...
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Hagman, Joel H, ...
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Jonasson, Jenny, ...
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Deschout, Hendri ...
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Bernin, Diana, 1 ...
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Cella-Zanacchi, ...
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show more...
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Diaspro, A.
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McNally, J.G.
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Ameloot, M.
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Smisdom, N.
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Nydén, Magnus, 1 ...
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Hermansson, Anne ...
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Rudemo, Mats, 19 ...
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Braeckmans, Kevi ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Materials Chemis ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Mathematics
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and Other Mathematic ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Other Physics To ...
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Quarterly Review ...
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Chalmers University of Technology
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RISE