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Disposable microfluidic chip with integrated light sheet illumination enables diagnostics based on membrane vesicles

Deschout, Hendrik (author)
Universiteit Gent,Ghent university
Raemdonck, K. (author)
Universiteit Gent,Ghent university
Stremersch, S. (author)
Universiteit Gent,Ghent university
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Maoddi, P. (author)
Ecole Polytechnique Federale de Lausanne (EPFL),Swiss Federal Institute of Technology in Lausanne (EPFL)
Mernier, G. (author)
Ecole Polytechnique Federale de Lausanne (EPFL),Swiss Federal Institute of Technology in Lausanne (EPFL)
Renaud, P. (author)
Ecole Polytechnique Federale de Lausanne (EPFL),Swiss Federal Institute of Technology in Lausanne (EPFL)
Jiguet, S. (author)
Ecole Polytechnique Federale de Lausanne (EPFL),Swiss Federal Institute of Technology in Lausanne (EPFL)
Hendrix, A. (author)
Universiteit Gent,Ghent university
Bracke, M. (author)
Universiteit Gent,Ghent university
Van den Broecke, R. (author)
Universiteit Gent,Ghent university
Röding, Magnus, 1984 (author)
Chalmers tekniska högskola,Chalmers University of Technology,Göteborgs universitet,University of Gothenburg
Rudemo, Mats, 1937 (author)
Göteborgs universitet,University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology
Demeester, J. (author)
Universiteit Gent,Ghent university
De Smedt, S. (author)
Universiteit Gent,Ghent university
Strubbe, F. (author)
Universiteit Gent,Ghent university
Neyts, K. (author)
Universiteit Gent,Ghent university
Braeckmans, Kevin (author)
Universiteit Gent,Ghent university
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 (creator_code:org_t)
2013
2013
English.
In: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013. ; 3, s. 2010-2012
  • Conference paper (peer-reviewed)
Abstract Subject headings
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  • Cell-derived membrane vesicles that are released in body fluids are emerging as potential non-invasive biomarkers for diseases like cancer. Techniques capable of measuring the size and concentration of such membrane vesicles directly in body fluids are urgently needed. Here we report on a microfluidic chip with integrated light sheet illumination, and demonstrate accurate fluorescence Single Particle Tracking measurements of the size and concentration of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours.

Subject headings

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Keyword

Diagnostics
Single Particle Tracking
Light sheet microscopy
Lab-on-a-chip

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