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A universal fixatio...
A universal fixation method based on quaternary ammonium salts (RNAlater) for omics-technologies: Saccharomyces cerevisiae as a case study
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- van Eijsden, R. G. E. (author)
- Flanders Interuniversity Institute for Biotechnology
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- Stassen, C. (author)
- Universiteit Gent,Ghent university
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- Daenen, L. (author)
- Katholieke Universiteit Leuven
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- Van Mulders, S. E. (author)
- Katholieke Universiteit Leuven
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- Bapat, P. M. (author)
- Danmarks Tekniske Universitet,Technical University of Denmark
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- Siewers, Verena, 1976 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Goossens, K. V. Y. (author)
- Vrije Universiteit Brüssel (VUB),Vrije Universiteit Brussel (VUB)
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- Nielsen, Jens B, 1962 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Delvaux, F. R. (author)
- Katholieke Universiteit Leuven
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- Van Hummelen, P. (author)
- Dana-Farber Cancer Institute
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- Devreese, B. (author)
- Universiteit Gent,Ghent university
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- Willaert, R. G. (author)
- Vrije Universiteit Brüssel (VUB),Vrije Universiteit Brussel (VUB)
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(creator_code:org_t)
- 2013-02-16
- 2013
- English.
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In: Biotechnology Letters. - : Springer Science and Business Media LLC. - 1573-6776 .- 0141-5492. ; 35:6, s. 891-900
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http://dx.doi.org/10...
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https://doi.org/10.1...
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Abstract
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- Genomics, transcriptomics, proteomics and fluxomics are powerful omics-technologies that play a major role in today's research. For each of these techniques good sample quality is crucial. Major factors contributing to the quality of a sample is the actual sampling procedure itself and the way the sample is stored directly after sampling. It has already been described that RNAlater can be used to store tissues and cells in a way that the RNA quality and quantity are preserved. In this paper, we demonstrate that quaternary ammonium salts (RNAlater) are also suitable to preserve and store samples from Saccharomyces cerevisiae for later use with the four major omics-technologies. Moreover, it is shown that RNAlater also preserves the cell morphology and the potential to recover growth, permitting microscopic analysis and yeast cell culturing at a later stage.
Subject headings
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Keyword
- Genomics
- Omics
- Fluxomics
- RNAlater
- Saccharomyces cerevisiae
- Transcriptomics
Publication and Content Type
- art (subject category)
- ref (subject category)
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van Eijsden, R. ...
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Stassen, C.
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Daenen, L.
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Van Mulders, S. ...
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Bapat, P. M.
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Siewers, Verena, ...
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show more...
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Goossens, K. V. ...
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Nielsen, Jens B, ...
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Delvaux, F. R.
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Van Hummelen, P.
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Devreese, B.
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Willaert, R. G.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Bioinformatics a ...
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Biotechnology Le ...
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Chalmers University of Technology