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Comparison of prote...
Comparison of proteomic responses as global approach to antibiotic mechanism of action elucidation
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- Senges, Christoph H.R. (author)
- Ruhr-Universität Bochum
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- Stepanek, Jennifer J. (author)
- Ruhr-Universität Bochum
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- Wenzel, Michaela, 1986 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Ruhr-Universität Bochum
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- Raatschen, Nadja (author)
- Ruhr-Universität Bochum
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- Ay, Ümran (author)
- Ruhr-Universität Bochum
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- Märtens, Yvonne (author)
- Ruhr-Universität Bochum
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- Prochnow, Pascal (author)
- Ruhr-Universität Bochum
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- Hernández, Melissa Vázquez (author)
- Ruhr-Universität Bochum
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- Yayci, Abdulkadir (author)
- Ruhr-Universität Bochum
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- Schubert, Britta (author)
- Ruhr-Universität Bochum
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- Janzing, Niklas B.M. (author)
- Ruhr-Universität Bochum
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- Warmuth, Helen L. (author)
- Ruhr-Universität Bochum
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- Kozik, Martin (author)
- Ruhr-Universität Bochum
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- Bongard, Jens (author)
- Ruhr-Universität Bochum
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- Alumasa, John N. (author)
- Pennsylvania State University
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- Albada, Bauke (author)
- Wageningen University and Research,Ruhr-Universität Bochum
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- Penkova, Maya (author)
- Ruhr-Universität Bochum
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Lukežič, Tadeja (author)
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- Sorto, Nohemy A. (author)
- University of California
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- Lorenz, Nicole (author)
- Ruhr-Universität Bochum
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- Miller, Reece G. (author)
- Ruhr-Universität Bochum
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- Zhu, Bingyao (author)
- Georg-August-Universität Göttingen,University of Göttingen
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- Benda, Martin (author)
- Georg-August-Universität Göttingen,University of Göttingen
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- Stülke, J. (author)
- Georg-August-Universität Göttingen,University of Göttingen
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- Schäkermann, Sina (author)
- Ruhr-Universität Bochum
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- Leichert, Lars I. (author)
- Ruhr-Universität Bochum
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Scheinpflug, Kathi (author)
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- Brötz-Oesterhelt, Heike (author)
- Eberhard Karls Universität Tübingen,Eberhard Karls University of Tübingen
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- Hertweck, C. (author)
- Hans-Knoll-Institute (HKI)
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- Shaw, Jared T. (author)
- University of California
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- Petković, Hrvoje (author)
- Univerza V Ljubljani,University of Ljubljana
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Brunel, Jean M. (author)
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- Keiler, Kenneth C. (author)
- Pennsylvania State University
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- Metzler-Nolte, Nils (author)
- Ruhr-Universität Bochum
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- Bandow, Julia E. (author)
- Ruhr-Universität Bochum
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(creator_code:org_t)
- 2021
- 2021
- English.
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In: Antimicrobial Agents and Chemotherapy. - 0066-4804 .- 1098-6596. ; 65:1
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https://research.cha...
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https://doi.org/10.1...
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Abstract
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- This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. New antibiotics are urgently needed to address the mounting resistance challenge. In early drug discovery, one of the bottlenecks is the elucidation of targets and mechanisms. To accelerate antibiotic research, we provide a proteomic approach for the rapid classification of compounds into those with precedented and unprecedented modes of action. We established a proteomic response library of Bacillus subtilis covering 91 antibiotics and comparator compounds, and a mathematical approach was developed to aid data analysis. Comparison of proteomic responses (CoPR) allows the rapid identification of antibiotics with dual mechanisms of action as shown for atypical tetracyclines. It also aids in generating hypotheses on mechanisms of action as presented for salvarsan (arsphenamine) and the antirheumatic agent auranofin, which is under consideration for repurposing. Proteomic profiling also provides insights into the impact of antibiotics on bacterial physiology through analysis of marker proteins indicative of the impairment of cellular processes and structures. As demonstrated for trans-translation, a promising target not yet exploited clinically, proteomic profiling supports chemical biology approaches to investigating bacterial physiology.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Physiology
- Antibiotic
- Chemical biology
- Mechanism of action
- Proteomics
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Senges, Christop ...
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Stepanek, Jennif ...
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Wenzel, Michaela ...
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Raatschen, Nadja
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Ay, Ümran
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Märtens, Yvonne
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show more...
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Prochnow, Pascal
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Hernández, Melis ...
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Yayci, Abdulkadi ...
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Schubert, Britta
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Janzing, Niklas ...
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Warmuth, Helen L ...
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Kozik, Martin
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Bongard, Jens
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Alumasa, John N.
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Albada, Bauke
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Penkova, Maya
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Lukežič, Tadeja
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Sorto, Nohemy A.
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Lorenz, Nicole
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Miller, Reece G.
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Zhu, Bingyao
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Benda, Martin
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Stülke, J.
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Schäkermann, Sin ...
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Leichert, Lars I ...
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Scheinpflug, Kat ...
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Brötz-Oesterhelt ...
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Hertweck, C.
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Shaw, Jared T.
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Petković, Hrvoje
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Brunel, Jean M.
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Keiler, Kenneth ...
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Metzler-Nolte, N ...
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Bandow, Julia E.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
- Articles in the publication
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Antimicrobial Ag ...
- By the university
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Chalmers University of Technology