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Adaptive laboratory...
Adaptive laboratory evolution of tolerance to dicarboxylic acids in Saccharomyces cerevisiae
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- Pereira, Rui, 1986 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Novo Nordisk Fonden,Novo Nordisk Foundation
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- Wei, Yongjun, 1986 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Mohamed, Elsayed (author)
- Danmarks Tekniske Universitet,Technical University of Denmark
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- Radi, Mohammad (author)
- Danmarks Tekniske Universitet,Technical University of Denmark
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- Malina, Carl, 1992 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Herrgard, M. J. (author)
- Danmarks Tekniske Universitet,Technical University of Denmark
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- Feist, Adam M. (author)
- University of California,Danmarks Tekniske Universitet,Technical University of Denmark
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- Nielsen, Jens B, 1962 (author)
- BioInnovation Institute (BII),Chalmers tekniska högskola,Chalmers University of Technology,Danmarks Tekniske Universitet,Technical University of Denmark
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- Chen, Yun, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- Elsevier BV, 2019
- 2019
- English.
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In: Metabolic Engineering. - : Elsevier BV. - 1096-7176 .- 1096-7184. ; 56, s. 130-141
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Abstract
Subject headings
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- Improving the growth phenotypes of microbes in high product concentrations is an essential design objective in the development of robust cell factories. However, the limited knowledge regarding tolerance mechanisms makes rational design of such traits complicated. Here, adaptive laboratory evolution was used to explore the tolerance mechanisms that Saccharomyces cerevisiae can evolve in the presence of inhibiting concentrations of three dicarboxylic acids: glutaric acid, adipic acid and pimelic acid. Whole-genome sequencing of tolerant mutants enabled the discovery of the genetic changes behind tolerance and most mutations could be linked to the up-regulation of multidrug resistance transporters. The amplification of QDR3, in particular, was shown to confer tolerance not only to the three dicarboxylic acids investigated, but also towards muconic acid and glutaconic acid. In addition to increased acid tolerance, QDR3 overexpression also improved the production of muconic acid in the context of a strain engineered for producing this compound.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Multidrug resistance transporter
- Dicarboxylic acid
- Adaptive laboratory evolution
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Pereira, Rui, 19 ...
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Wei, Yongjun, 19 ...
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Mohamed, Elsayed
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Radi, Mohammad
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Malina, Carl, 19 ...
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Herrgard, M. J.
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show more...
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Feist, Adam M.
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Nielsen, Jens B, ...
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Chen, Yun, 1978
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Other Basic Medi ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
- Articles in the publication
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Metabolic Engine ...
- By the university
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Chalmers University of Technology