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DNA-BINDING AND PHO...
DNA-BINDING AND PHOTOCLEAVAGE BY URANYL(VI) (UO2(2+)) SALTS
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Nielsen, P. E. (author)
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- Hiort, Catharina, 1962 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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Sonnichsen, S. H. (author)
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Buchardt, O. (author)
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Dahl, O. (author)
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- Nordén, Bengt, 1945 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2002-05-01
- 1992
- English.
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In: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 1520-5126 .- 0002-7863. ; 114:13, s. 4967-4975
- Related links:
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http://dx.doi.org/10...
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The interaction of the uranyl(VI) ion (UO22+) with DNA and its light-induced cleavage of DNA has been studied using flow-linear dichroism and P-32-endlabeled oligonucleotides. It was found that binding of uranyl ion to DNA is a prerequisite for photocleavage; from run-off experiments the binding constant was estimated to be of the order of 10(10) M-1 at pH 4. The angular orientation of the [O=U=O]2+ chromophore is consistent with binding by bridging phosphate groups on opposite strands of the minor groove of DNA; at higher DNA concentration aggregation indicates intermolecular bridging as well. The uranyl-mediated photocleavage of DNA is not influenced by the presence of O2, is more efficient at low pH (
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- reduced dichroism curves
- dichroism
- mechanisms
- linear
- 1
- contacts
- degradation
- release
- identification
- nuclease activity
- 10-phenanthroline copper-ion
- complex
Publication and Content Type
- art (subject category)
- ref (subject category)
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