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Microfluidic gradient-generating device for pharmacological profiling
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- Pihl, Johan, 1975 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Sinclair, J. (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Sahlin, Eskil, 1968 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
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- Karlsson, Mattias, 1980 (author)
- Petterson, F. (author)
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- (creator_code:org_t)
- 2005-05-26
- 2005
- English.
- In: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 77:13, s. 3897-3903
- Journal article (peer-reviewed)
Abstract
Subject headings
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- We describe an on-chip microfluidic gradient-generating device that generates concentration gradients spanning nearly 5 orders of magnitude starting from a single concentration. The exiting stream of drugs held at different concentrations remains laminar in a recording chamber and can be presented as 24 discrete solutions to a cell-based sensor. The high-performance characteristics of the device are demonstrated by pharmacological screening of voltage-gated K + channels (hERG) and ligand-gated GABAA receptors using scanning-probe patch-clamp measurements. Multiple data point dose-response curves and IC 50 and EC 50 values were rapidly obtained, typically in less than 30 min, through its combined functionality of gradient generation and open-volume laminar flow. The device facilitates rapid pharmacological profiling of ion channel and GPCR effectors and enables the acquisition of large numbers of data points with minute sample consumption and handling. © 2005 American Chemical Society.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- poly(dimethylsiloxane)
- systems
- cells
- drug discovery
- channel
- xenopus oocytes
- receptors
- xenopus oocytes
- drug discovery
- channel
- systems
- cells
- poly(dimethylsiloxane)
- receptors
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- art (subject category)
- ref (subject category)
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- Orwar, Owe, 1964
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