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Microfluidic gradie...
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Pihl, Johan,1975Chalmers tekniska högskola,Chalmers University of Technology
(author)
Microfluidic gradient-generating device for pharmacological profiling
- Article/chapterEnglish2005
Publisher, publication year, extent ...
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2005-05-26
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American Chemical Society (ACS),2005
Numbers
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LIBRIS-ID:oai:research.chalmers.se:a0170855-e30d-475f-9a5d-96074df72db5
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https://research.chalmers.se/publication/80542URI
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https://doi.org/10.1021/ac050218+DOI
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https://gup.ub.gu.se/publication/80542URI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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We describe an on-chip microfluidic gradient-generating device that generates concentration gradients spanning nearly 5 orders of magnitude starting from a single concentration. The exiting stream of drugs held at different concentrations remains laminar in a recording chamber and can be presented as 24 discrete solutions to a cell-based sensor. The high-performance characteristics of the device are demonstrated by pharmacological screening of voltage-gated K + channels (hERG) and ligand-gated GABAA receptors using scanning-probe patch-clamp measurements. Multiple data point dose-response curves and IC 50 and EC 50 values were rapidly obtained, typically in less than 30 min, through its combined functionality of gradient generation and open-volume laminar flow. The device facilitates rapid pharmacological profiling of ion channel and GPCR effectors and enables the acquisition of large numbers of data points with minute sample consumption and handling. © 2005 American Chemical Society.
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Sinclair, J.Chalmers tekniska högskola,Chalmers University of Technology
(author)
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Sahlin, Eskil,1968Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
(author)
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Karlsson, Mattias,1980(Swepub:cth)makarl
(author)
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Petterson, F.
(author)
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Olofsson, Jessica,1975Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)jessicao
(author)
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Orwar, Owe,1964Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)orwar
(author)
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Chalmers tekniska högskolaInstitutionen för kemi
(creator_code:org_t)
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In:Analytical Chemistry: American Chemical Society (ACS)77:13, s. 3897-39030003-27001520-6882
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Pihl, Johan, 197 ...
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Sinclair, J.
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Sahlin, Eskil, 1 ...
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Karlsson, Mattia ...
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Petterson, F.
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Olofsson, Jessic ...
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Orwar, Owe, 1964
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- NATURAL SCIENCES
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NATURAL SCIENCES
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Analytical Chemi ...
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Chalmers University of Technology
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University of Gothenburg