Search: onr:"swepub:oai:research.chalmers.se:a0e398d4-531d-464e-a942-1f71f89e96e1" >
PNA HYBRIDIZES TO C...
PNA HYBRIDIZES TO COMPLEMENTARY OLIGONUCLEOTIDES OBEYING THE WATSON-CRICK HYDROGEN-BONDING RULES
-
Egholm, M. (author)
-
Buchardt, O. (author)
-
Christensen, L. (author)
-
show more...
-
Behrens, C. (author)
-
Freier, S. M. (author)
-
Driver, D. A. (author)
-
Berg, R. H. (author)
-
- Kim, Seog K. (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Nordén, Bengt, 1945 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
Nielsen, P. E. (author)
-
show less...
-
(creator_code:org_t)
- Springer Science and Business Media LLC, 1993
- 1993
- English.
-
In: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 365:6446, s. 566-568
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://research.cha...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- DNA ANALOGUES are currently being intensely investigated owing to their potential as gene-targeted drugs1-3. Furthermore, their properties and interaction with DNA and RNA could provide a better understanding of the structural features of natural DNA that determine its unique chemical, biological and genetic properties3,4. We recently designed a DNA analogue, PNA, in which the backbone is structurally homomorphous with the deoxyribose backbone and consists of N-(2-aminoethyl)glycine units to which the nucleobases are attached5-9. We showed that PNA oligomers containing solely thymine and cytosine can hybridize to complementary oligonucleotides, presumably by forming Watson-Crick-Hoogsteen (PNA)2-DNA triplexes, which are much more stable than the corresponding DNA-DNA duplexes5-7, and bind to double-stranded DNA by strand displacement5,8. We report here that PNA containing all four natural nucleobases hybridizes to complementary oligonucleotides obeying the Watson-Crick base-pairing rules, and thus is a true DNA mimic in terms of base-pair recognition.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- dna
- peptide nucleic-acids
- thymine
- recognition
- antisense
- stability
- replication
- helices
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
-
Nature
(Search for host publication in LIBRIS)
To the university's database
- By the author/editor
-
Egholm, M.
-
Buchardt, O.
-
Christensen, L.
-
Behrens, C.
-
Freier, S. M.
-
Driver, D. A.
-
show more...
-
Berg, R. H.
-
Kim, Seog K.
-
Nordén, Bengt, 1 ...
-
Nielsen, P. E.
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Chemical Science ...
- Articles in the publication
-
Nature
- By the university
-
Chalmers University of Technology