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  • Schmitt, AndreasUmeå universitet,Institutionen för medicinsk kemi och biofysik,Umeå University (author)

PrgB promotes aggregation, biofilm formation, and conjugation through DNA binding and compaction

  • Article/chapterEnglish2018

Publisher, publication year, extent ...

  • 2018-07-31
  • Wiley,2018

Numbers

  • LIBRIS-ID:oai:research.chalmers.se:a19d0a5a-147b-4e01-ab48-d3bc02cfd283
  • https://doi.org/10.1111/mmi.13980DOI
  • https://research.chalmers.se/publication/504944URI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-152231URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Gram-positive bacteria deploy type IV secretion systems (T4SSs) to facilitate horizontal gene transfer. The T4SSs of Gram-positive bacteria rely on surface adhesins as opposed to conjugative pili to facilitate mating. Enterococcus faecalis PrgB is a surface adhesin that promotes mating pair formation and robust biofilm development in an extracellular DNA (eDNA) dependent manner. Here, we report the structure of the adhesin domain of PrgB. The adhesin domain binds and compacts DNA in vitro. In vivo PrgB deleted of its adhesin domain does not support cellular aggregation, biofilm development and conjugative DNA transfer. PrgB also binds lipoteichoic acid (LTA), which competes with DNA binding. We propose that PrgB binding and compaction of eDNA facilitates cell aggregation and plays an important role in establishment of early biofilms in mono- or polyspecies settings. Within these biofilms, PrgB mediates formation and stabilization of direct cell-cell contacts through alternative binding of cell-bound LTA, which in turn promotes establishment of productive mating junctions and efficient intra- or inter-species T4SS-mediated gene transfer.

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Added entries (persons, corporate bodies, meetings, titles ...)

  • Jiang, Kai,1988Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)jkai (author)
  • Camacho, Martha I. (author)
  • Jonna, Venkateswara RaoUmeå universitet,Institutionen för medicinsk kemi och biofysik,Umeå University(Swepub:umu)vera0003 (author)
  • Hofer, AndersUmeå universitet,Institutionen för medicinsk kemi och biofysik,Umeå University(Swepub:umu)anho0002 (author)
  • Westerlund, Fredrik,1978Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)fredrikw (author)
  • Christie, Peter J. (author)
  • Berntsson, Ronnie P-A.Umeå universitet,Institutionen för medicinsk kemi och biofysik,Umeå University(Swepub:umu)robe0049 (author)
  • Umeå universitetInstitutionen för medicinsk kemi och biofysik (creator_code:org_t)

Related titles

  • In:Molecular Microbiology: Wiley109:3, s. 291-3051365-29580950-382X

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