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Deleting the para-n...
Deleting the para-nitrophenyl phosphatase (pNPPase), PHO13, in recombinant Saccharomyces cerevisiae improves growth and ethanol production on D-xylose
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Headman Van Vleet, Jennifer (author)
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Jeffries, Thomas W. (author)
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Olsson, Lisbeth, 1963 (author)
- 2008
- 2008
- English.
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In: Metabolic Engineering. - 1096-7176 .- 1096-7184. ; :10, s. 360-369
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Abstract
Subject headings
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- Overexpression of D-xylulokinase in Saccharomyces cerevisiae engineered for assimilation of xylose results in growth inhibition that is more pronounced at higher xylose concentrations. Mutants deficient in the para-nitrophenyl phosphatase, PHO13, resist growth inhibition on xylose. We studied this inhibition under aerobic growth conditions in well-controlled bioreactors using engineered S. cerevisiae CEN.PK. Growth on glucose was not significantly affected in pho13∆ mutants, but acetate production increased by 75%. Cell growth, ethanol production, and xylose consumption all increased markedly in pho13∆ mutants. The specific growth rate and rate of specific xylose uptake were approximately 1.5 times higher in the deletion strain than in the parental strain when growing on glucose-xylose mixtures and up to 10-fold higher when growing on xylose alone. In addition to showing higher acetate levels, pho13∆ mutants also produced less glycerol on xylose, suggesting that deletion of Pho13p could improve growth by altering redox levels when cells are grown on xylose.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Keyword
- Xylose
- XYL3
- XYL1
- Fermentation
- Xylulokinase
- XYL2
- PHO13
- XKS1
- Overexpression
Publication and Content Type
- art (subject category)
- ref (subject category)
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