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Rewiring carbon flu...
Rewiring carbon flux in Escherichia coli using a bifunctional molecular switch
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- Hou, Jianshen (author)
- Jiangnan University
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- Gao, Cong (author)
- Jiangnan University
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- Guo, Liang (author)
- Jiangnan University
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- Nielsen, Jens B, 1962 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Ding, Qiang (author)
- Jiangnan University
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- Tang, Wenxiu (author)
- Jiangnan University
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- Hu, Guipeng (author)
- Jiangnan University
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- Chen, Xiulai (author)
- Jiangnan University
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- Liu, Liming, 1976 (author)
- Jiangnan University
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(creator_code:org_t)
- Elsevier BV, 2020
- 2020
- English.
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In: Metabolic Engineering. - : Elsevier BV. - 1096-7176 .- 1096-7184. ; 61, s. 47-57
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Abstract
Subject headings
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- The unbalanced distribution of carbon flux in microbial cell factories can lead to inefficient production and poor cell growth. Uncoupling cell growth and chemical synthesis can therefore improve microbial cell factory efficiency. Such uncoupling, which requires precise manipulation of carbon fluxes, can be achieved by up-regulating or down-regulating the expression of enzymes of various pathways. In this study, a dynamic turn-off switch (dTFS) and a dynamic turn-on switch (dTNS) were constructed using growth phase-dependent promoters and degrons. By combining the dTFS and dTNS, a bifunctional molecular switch that could orthogonally regulate two target proteins was introduced. This bifunctional molecular switch was used to uncouple cell growth from shikimic acid and D-glucaric acid synthesis, resulting in the production of 14.33 g/L shikimic acid and the highest reported productivity of D-glucaric acid (0.0325 g/L/h) in Escherichia coli MG1655. This proved that the bifunctional molecular switch could rewire carbon fluxes by controlling target protein abundance.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- Metabolic engineering
- Dynamic regulation
- Synthetic biology
- Metabolic flux regulation
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Hou, Jianshen
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Gao, Cong
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Guo, Liang
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Nielsen, Jens B, ...
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Ding, Qiang
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Tang, Wenxiu
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show more...
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Hu, Guipeng
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Chen, Xiulai
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Liu, Liming, 197 ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
- Articles in the publication
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Metabolic Engine ...
- By the university
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Chalmers University of Technology