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The impact of respi...
The impact of respiration and oxidative stress response on recombinant ?-amylase production by Saccharomyces cerevisiae
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- Martinez Ruiz, Jose Luis, 1981 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Meza, Eugenio, 1975 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Petranovic Nielsen, Dina, 1975 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Nielsen, Jens B, 1962 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- Elsevier BV, 2016
- 2016
- English.
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In: Metabolic Engineering Communications. - : Elsevier BV. - 2214-0301. ; 3, s. 205-210
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Abstract
Subject headings
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- Studying protein production is important for fundamental research on cell biology and applied research for biotechnology. Yeast Saccharomyces cerevisiae is an attractive workhorse for production of recombinant proteins as it does not secrete many endogenous proteins and it is therefore easy to purify a secreted product. However, recombinant production at high rates represents a significant metabolic burden for the yeast cells, which results in oxidative stress and ultimately affects the protein production capacity. Here we describe a method to reduce the overall oxidative stress by overexpressing the endogenous HAP1 gene in a S. cerevisiae strain overproducing recombinant α-amylase. We demonstrate how Hap1p can activate a set of oxidative stress response genes and meanwhile contribute to increase the metabolic rate of the yeast strains, therefore mitigating the negative effect of the ROS accumulation associated to protein folding and hence increasing the production capacity during batch fermentations.
Subject headings
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Keyword
- Amylase
- Hap1
- Oxidative stress response
- Protein production
- Yeast
Publication and Content Type
- art (subject category)
- ref (subject category)
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